An EST library from Puccinia graminis f. sp tritici reveals genes potentially involved in fungal differentiation
An EST library from Puccinia graminis f. sp tritici reveals genes potentially involved in fungal differentiation
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DOI：
10.1111/j.1574-6968.2006.00127.x
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发表时间：
2006-03-01
影响因子：
2.1
通讯作者：
Moerschbacher, BM
中科院分区：
文献类型：
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作者：
Broeker, K;Bernard, F;Moerschbacher, BM
小麦锈菌Puccinia graminis f.小麦赤霉病菌是小麦植株上的专性生物营养型病原体，因此难以调查。因此，在分子水平上对这种真菌知之甚少。我们构建了一个差异抑制消减杂交cDNA文库，从锈病感染与健康小麦植株。大多数表达序列标签（EST）显示与真菌序列的相似性。半定量RT-PCR使用的mRNA从锈病感染的叶子，从axenically生长，分化和nondifferentiating年轻的锈病菌落，以及孢子形成和nonsporulating成熟菌丝体揭示了相当不同的表达模式，不同的EST，脱落的新的光在分化和宿主-病原体相互作用的潜在参与。
The rust fungus Puccinia graminis f. sp. tritici is an obligately biotrophic pathogen on wheat plants and thus difficult to investigate. Hence, little is known about this fungus at the molecular level. We constructed a differential suppression subtractive hybridization cDNA-library from rust-infected vs. healthy wheat plants. The majority of expressed sequence tags (ESTs) showed similarities to fungal sequences. Semiquantitative RT-PCR using mRNA from rust-infected leaves, and from axenically grown, differentiating and nondifferentiating young rust colonies as well as sporulating and nonsporulating mature mycelia revealed rather diverse expression patterns for different ESTs, shedding new light on their potential involvement in differentiation and host-pathogen interaction.