Glucose-dependent insulinotropic peptide receptor expression in the hippocampus and neocortex of mesial temporal lobe epilepsy patients and rats undergoing pilocarpine induced status epilepticus
Glucose-dependent insulinotropic peptide receptor expression in the hippocampus and neocortex of mesial temporal lobe epilepsy patients and rats undergoing pilocarpine induced status epilepticus
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DOI：
10.1016/j.peptides.2010.12.010
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发表时间：
2011-04-01
期刊：
影响因子：
3
通讯作者：
Walz, Roger
中科院分区：
文献类型：
--
作者：
Figueiredo, Claudia P.;Antunes, Victor L. S.;Walz, Roger
葡萄糖依赖性促胰岛素肽受体（GIPR）与神经可塑性有关，可能与癫痫有关。采用免疫组织化学方法分析了匹罗卡品致癫痫持续状态（Pilo-SE）大鼠海马（HIP）和新皮质（Cx）中GIPR的表达，并对3例经手术治疗的与海马硬化相关的左侧内侧颞叶癫痫（MTI.E-HS）的年轻男性患者进行了GIPR表达分析。采用免疫组化Gin法和Fluoro-Jade法观察GIPR表达与Pilo-SE诱导的神经元变性之间的关系。GIPR在动物和人的海马CA亚区、齿状回（DG）和Cx的神经元细胞质中表达。Pilo-SE诱导后，GIPR表达在HIP中在1 h和5 d后显著增加，但在12 h或50 d后不显著。在Cx中，GIPR表达在Pilo-SE后1 h、12 h和5 d增加，但在50 d后不增加。Pilo-SE后12 h GIPR的表达与Fluoro-Jade染色强度成反比。在人体组织中，GIPR表达模式与慢性Pilo-SE动物中观察到的相似。在人体样本中未观察到Fluoro-Jade染色细胞。GIPR在人HIP和Cx中表达。Pilo-SE后，HIP和Cx中GIPR表达呈时间和区域依赖性增加，与神经元变性呈负相关。(C)2010年爱思唯尔公司All rights reserved.
The glucose-dependent insulinotropic peptide receptor (GIPR) has been implicated with neuroplasticity and may be related to epilepsy. GIPR expression was analyzed by immunohistochemistry in the hippocampus (HIP) and neocortex (Cx) of rats undergoing pilocarpine induced status epilepticus (Pilo-SE), and in three young male patients with left mesial temporal lobe epilepsy related to hippocampal sclerosis (MTI.E-HS) treated surgically. A combined Gin immunohistochemistry and Fluoro-Jade staining was carried out to investigate the association between the GIPR expression and neuronal degeneration induced by Pilo-SE. GIPR was expressed in the cytoplasm of neurons from the HIP CA subfields, dentate gyrus (DG) and Cx of animals and human samples. The GIPR expression after the Pilo-SE induction increases significantly in the HIP after 1 h and 5 days, but not after 12 h or 50 days. In the Cx, the GIPR expression increases after 1 h, 12 h and 5 days, but not 50 days after the Pilo-SE. The expression of GIPR 12 h after Pilo-SE was inversely proportional to the Fluoro-Jade staining intensity. In the human tissue, GIPR expression patterns were similar to those observed in chronic Pilo-SE animals. No Fluoro-Jade stained cells were observed in the human sample. GIPR is expressed in human HIP and Cx. There was a time and region dependent increase of GIPR expression in the HIP and Cx after Pilo-SE that was inversely associated to neuronal degeneration. (C) 2010 Elsevier Inc. All rights reserved.