Profiling the Response of Human Hair Follicles to Ultraviolet Radiation
Profiling the Response of Human Hair Follicles to Ultraviolet Radiation
复制标题
DOI：
10.1038/jid.2008.418
复制
发表时间：
2009-07-01
影响因子：
6.5
通讯作者：
Paus, Ralf
中科院分区：
文献类型：
--
作者：
Lu, Zhongfa;Fischer, Tobias W.;Paus, Ralf
过量的紫外线辐射是对人体皮肤最有害的环境影响之一。然而，紫外线对人体皮肤附属器的直接影响仍有待系统研究。对体外培养的人毛发生长初期毛囊进行照射，用UVB（20/50 mJ cm（-2））照射后，观察到毛干伸长减少、过早进入退化期和毛基质角质形成细胞增殖减少。在20 mJ cm（-2）时，凋亡细胞死亡占主导地位（casp-3/p53激活），而在50 mJ cm（-2）时，坏死细胞死亡占主导地位（乳酸脱氢酶增加）。线粒体常见缺失和氧化损伤的基因组DNA（8-OH-dG）主要在20 mJ cm（-2）处观察到。滤泡黑素生成和ACTH免疫反应性急剧下降，但α-黑素细胞刺激激素保持不变，而转化生长因子β 2表达从外转向内根鞘。Giemsa+肥大细胞的数量和肥大细胞脱粒的程度增加，在结缔组织鞘（CTS），和CTS细胞和基质角质形成细胞的CD 117免疫反应性上调。因此，紫外线辐射差异修改头发的生长和周期，促进细胞死亡，并诱导复杂的调控事件在体外人类毛囊。从这个人体器官模型，这是一个活的和人体组织的相互作用系统，在生理相关的原位条件下，可能会鼓励其用于一般调查的紫外线诱导的影响，以及用于测试可能的代理人的紫外线保护效力。
Excessive UVR ranks among the most harmful environmental influences on human skin. However, the direct impact of UVR on human skin appendages remains to be systematically investigated. Organ-cultured human anagen hair follicles in vitro were irradiated, and reduction of hair shaft elongation, premature catagen entry, and reduced hair matrix keratinocyte proliferation were observed upon irradiation with UVB (20/50 mJ cm(-2)). At 20 mJ cm(-2), apoptotic cell death prevailed (casp-3/p53 activation), whereas at 50mJ cm(-2), necrotic cell death was predominant ( lactate dehydrogenase increase). Mitochondrial common deletion and oxidatively damaged genomic DNA (8-OH-dG) was mainly observed at 20 mJ cm(-2). Follicular melanogenesis and ACTH immunoreactivity drastically declined, but alpha-melanocyte-stimulating hormone remained unchanged, whereas transforming growth factor-beta 2 expression shifted from the outer toward the inner root sheath. Both the number of Giemsa+ mast cells and the degree of mast-cell degranulation increased in the connective tissue sheath (CTS), and CD117 immunoreactivity of CTS cells and matrix keratinocytes was upregulated. Thus, UVR differentially modifies hair growth and cycle, promotes cell death, and induces complex regulatory events in human hair follicles in vitro. The leads from this human organ model, which is a living and human tissue interaction system under physiologically relevant in situ conditions, may encourage its use for general investigation of UV-induced effects as well as for testing possible agents for their UV-protective potency.