Measurement of rapamycin in whole blood using reverse-phase high-performance liquid chromatography.
Measurement of rapamycin in whole blood using reverse-phase high-performance liquid chromatography.
复制标题
使用反相高效液相色谱法测定全血中的雷帕霉素。
DOI：
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发表时间：
1992
影响因子：
2.5
通讯作者：
P. Bolingbroke
中科院分区：
文献类型：
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作者：
R. Yatscoff;C. Faraci;P. Bolingbroke
雷帕霉素(RapA)是一种免疫抑制剂，效力与FK 506相似，比环孢素高100倍。到目前为止，还没有关于该药物的药代动力学和处置的数据，主要是由于缺乏适当的分析方法。我们在这里描述了一种反相高效液相色谱(HPLC)全血中药物分析方法的评价。该方法具有良好的分析回收率，接近100%。方法的灵敏度为1.0微克/L，批间变异系数分别为14.4%和9.8%(10和50 mg/L)。结果发现，该药物在4℃下可稳定30天，在-40℃下可稳定55天。
Rapamycin (RAPA) is an immunosuppressant with a potency similar to FK 506 and up to 100-fold greater than cyclosporine. To date, no data have been reported on the pharmacokinetics and disposition of the drug, primarily due to a lack of suitable methods for its analysis. We describe here an evaluation of a reverse-phase high-performance liquid chromatographic (HPLC) method for analysis of the drug in whole blood. The method showed excellent analytical recovery of the drug, close to 100%. The sensitivity method was found to be 1.0 micrograms/L with between-run coefficients of variation of 14.4% and 9.8% at 10 and 50 mg/L. The stability of RAPA was investigated with this method in whole-blood specimens. It was found that the drug was stable for 30 days at 4 degrees C and for 55 days at -40 degrees C.