Proteomic Analysis of a Rice Mutant sd58 Possessing a Novel d1 Allele of Heterotrimeric G Protein Alpha Subunit (RGA1) in Salt Stress with a Focus on ROS Scavenging
Proteomic Analysis of a Rice Mutant sd58 Possessing a Novel d1 Allele of Heterotrimeric G Protein Alpha Subunit (RGA1) in Salt Stress with a Focus on ROS Scavenging
复制标题
水稻突变体 sd58 的蛋白质组学分析，该突变体具有异三聚 G 蛋白 Alpha 亚基 (RGA1) 的新型 d1 等位基因，在盐胁迫下重点关注 ROS 清除
DOI：
10.3390/ijms20010167
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发表时间：
2019-01-01
影响因子：
5.6
通讯作者：
Wang, Juan
中科院分区：
文献类型：
--
作者：
Peng, Peng;Gao, Yadi;Wang, Juan
在农业生产中，高盐严重抑制植物生长，导致农作物减产。因此，寻找植物抗盐的关键调控因子具有重要意义。本文报道了从甲基磺酸乙酯（EMS）诱变水稻突变体库中筛选到的一个新的耐盐性和矮秆表型突变体sd 58。遗传分析表明，sd 58为单隐性遗传。图位克隆和等位基因检测结果表明，sd 58的表型是由于编码异源三聚体G蛋白（Gα）α亚基的RGA 1突变所致。在RGA 1的第一内含子的剪接位点（GT-AG）处鉴定出点突变（G至A），其引起异常mRNA剪接形式的产生。此外，332差异丰富的蛋白质（DAP）确定通过使用等压标签相对和绝对定量（iTRAQ）为基础的蛋白质组学技术从幼苗的sd 58和Kitaake在盐处理。基因本体（GO）和KEGG途径富集分析表明，这些蛋白主要参与代谢途径、光合作用和活性氧（ROS）稳态等过程的调控。在盐胁迫下，sd 58表现出较低的ROS积累比Kitaake，这是与较高的酶活性参与ROS清除。因此，RGA 1可能通过清除ROS参与盐胁迫反应，这对阐明水稻异源三聚体G蛋白的耐盐机制具有重要意义。
High salinity severely restrains plant growth and results in decrease of crop yield in agricultural production. Thus, it is of great significance to discover the crucial regulators involved in plant salt resistance. Here, we report a novel mutant, sd58, which displays enhanced salt tolerance and dwarf phenotype, by screening from ethyl methane sulfonate (EMS) mutagenized rice mutant library. Genetic analysis showed that sd58 was caused by a single recessive locus. Map-based cloning and allelic test revealed that the phenotypes of sd58 were due to the mutation of RGA1, encoding the alpha subunit of heterotrimeric G protein (Gα). A point mutation (G to A) was identified at the splicing site (GT-AG) of the first intron in RGA1, which gives rise to the generation of abnormal mRNA splicing forms. Furthermore, 332 differentially abundant proteins (DAPs) were identified by using an Isobaric Tags for Relative and Absolute Quantitation(iTRAQ)-based proteomic technique from seedlings of sd58 and Kitaake in response to salt treatment. Gene Ontology (GO) and KEGG pathway enrichment analysis revealed these proteins were mainly involved in regulation of the processes such as metabolic pathways, photosynthesis and reactive oxygen species (ROS) homeostasis. Under salt stress, sd58 displayed lower ROS accumulation than Kitaake, which is consistent with the higher enzyme activities involved in ROS scavenging. Taken together, we propose that RGA1 is one of the regulators in salt response partially through ROS scavenging, which might be helpful in elucidating salt tolerant mechanisms of heterotrimeric G protein in rice.