PROTEIN-SPECIFIC HELPER LYMPHOCYTE-T FORMATION INITIATED BY DENDRITIC CELLS
PROTEIN-SPECIFIC HELPER LYMPHOCYTE-T FORMATION INITIATED BY DENDRITIC CELLS
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DOI：
10.1126/science.3160115
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
STEINMAN, RM
中科院分区：
文献类型：
--
作者：
INABA, K;STEINMAN, RM
对半抗原-多肽缀合物的抗体应答需要肽特异性辅助T细胞。后者可以通过提供少量树突细胞在组织培养中引发。然后，在明显不存在树突状细胞的情况下，经引发、辐照的辅助性T细胞诱导B细胞生长和分化。抗体反应的两个阶段-树突状细胞诱导辅助性T淋巴母细胞和将辅助性T淋巴母细胞从T细胞传递到B细胞-发生在离散的细胞聚集体中，这些细胞聚集体可以通过速度沉降分离。如果辅助性T母细胞恢复为较小的“记忆”淋巴细胞，则再次需要树突状细胞来启动抗体应答。
Antibody responses to hapten-polypeptide conjugates require peptide-specific helper T cells. The latter can be primed in tissue culture by providing small numbers of dendritic cells. Primed, irradiated helper T cells then induce B-cell growth and differentiation in the apparent absence of dendritic cells. Both stages of the antibody response—the induction of helper T lymphoblasts by dendritic cells and the delivery of help from T to B cell—occur in discrete cell aggregates that can be isolated by velocity sedimentation. If helper T blasts revert to smaller "memory" lymphocytes, dendritic cells again are needed to initiate the antibody response.