DNA MISMATCH CORRECTION IN A DEFINED SYSTEM
DNA MISMATCH CORRECTION IN A DEFINED SYSTEM
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DOI：
10.1126/science.2665076
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发表时间：
1989-07-14
期刊：
影响因子：
56.9
通讯作者：
MODRICH, P
中科院分区：
文献类型：
--
作者：
LAHUE, RS;AU, KG;MODRICH, P
DNA错配修复是一个链特异性过程，涉及对非互补的沃森 - 克里克核苷酸对的识别以及相距较远的DNA位点的参与。大肠杆菌的甲基导向反应已在一个由MutH、MutL和MutS蛋白、DNA解旋酶II、单链DNA结合蛋白、DNA聚合酶III全酶、核酸外切酶I、DNA连接酶以及ATP（三磷酸腺苷）和四种脱氧核苷三磷酸组成的纯化系统中重建。这组蛋白质能够在一个链特异性反应中处理八种碱基 - 碱基错配中的七种，该反应由距离错配位点1千碱基处的单个d(GATC)序列的甲基化状态所引导。
DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.