Rapid Flow Through Immunoassay for CRP Determination Based on Polyethylene Filters Modified with ω-Aminocellulose Carbamate
Rapid Flow Through Immunoassay for CRP Determination Based on Polyethylene Filters Modified with ω-Aminocellulose Carbamate
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DOI：
10.1002/mabi.201400179
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发表时间：
2014-11-01
影响因子：
4.6
通讯作者：
Heinze, Thomas
中科院分区：
文献类型：
--
作者：
Elschner, Thomas;Scholz, Friedrich;Heinze, Thomas
本文讨论了一种新的抗体固定化方法，并将其应用于柱基流通免疫分析系统（ABICAP）。结果表明，多孔聚乙烯材料可以用不同的ω-氨基纤维素氨基甲酸酯进行改性，得到用于抗体固定的含氨基的生物相容性支持物。抗hCRP抗体可以通过各种同双功能交联剂共价结合到表面。在夹心ABICAP系统中验证了抗体修饰的过滤器用于CRP测定。在基于胶体染料颗粒的10 min测试程序中，检测限为5 ng CRP mL（-1），变异系数为
A novel method for antibody immobilization is discussed and applied in a column based flow through immunoassay system (ABICAP). It is shown that porous polyethylene material can be modified with different omega-aminocellulose carbamates yielding an amino group containing biocompatible support for antibody immobilization. Anti-h CRP antibodies can be bound covalently to the surface via various homobifunctional cross-linkers. The antibody modified filters are validated for the CRP determination in a sandwich ABICAP system. In a 10 min test procedure based on colloidal dye particles, a limit of detection of 5 ng CRP mL(-1) and coefficients of variation of