Description of a fugu CXC chemokine and two CXC receptor genes, and characterization of the effects of different stimulators on their expression.
Description of a fugu CXC chemokine and two CXC receptor genes, and characterization of the effects of different stimulators on their expression.
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DOI：
10.1016/j.fsi.2007.06.008
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发表时间：
2007-12
影响因子：
4.7
通讯作者：
N. Saha;J. Bei;H. Suetake;Kyosuke Araki;W. Kai;K. Kikuchi;Hao-ran Lin;Yuzuru Suzuki
中科院分区：
文献类型：
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作者：
N. Saha;J. Bei;H. Suetake;Kyosuke Araki;W. Kai;K. Kikuchi;Hao-ran Lin;Yuzuru Suzuki
一个CXC趋化因子（CXCL 8）和两个CXC受体（CXCR）的一级结构已在东方鲀（Takifugu rubripes）中得到表征。与哺乳动物和鸟类不同，包括河豚在内的硬骨鱼类的CXCL 8缺乏ELR基序，该基序在中性粒细胞上的配体/受体相互作用中似乎很重要。基因组结构分析表明，河豚CXCL 8基因由4个外显子和3个内含子组成。与其他脊椎动物一样，从河豚中分离的两个CXCR基因编码具有特征性七个跨膜结构域的蛋白质CXCR 1和CXCR 2。每个受体由两个外显子组成，外显子之间由一个内含子隔开。同线性分析表明，这两个CXCRs来自硬骨鱼类的全基因组复制，不同于哺乳动物CXCR 1和CXCR 2。所有这些基因主要在淋巴组织中表达。PHA免疫刺激显示，PBL中CXCL 8和CXCRs的表达即使在很短的时间内也上调，但LPS刺激下调，这意味着这些基因参与了河豚免疫应答的调节。
The primary structures of a CXC chemokine (CXCL8) and two CXC receptors (CXCR) have been characterized in fugu, Takifugu rubripes. Unlike mammalian and avian species, CXCL8 of teleosts including fugu lacks the ELR motif that appears to be important in ligand/receptor interactions on neutrophils. Genomic organization shows that fugu CXCL8 gene consists of four exons and three introns. As in other vertebrates, two CXCR genes isolated from fugu encode proteins CXCR1 and CXCR2 that possess characteristic seven transmembrane domains. Each receptor consists of two exons separated by an intron. Synteny analysis indicates that these two CXCRs were derived from whole genome duplication in teleosts, differing from mammalian CXCR1 and CXCR2. All of these genes are primarily expressed in the lymphoid tissues. Immune stimulation with PHA showed that the expression of both CXCL8 and CXCRs in PBL are upregulated even after only a short time period, but downregulated by LPS stimulation, implying that these genes are involved in the regulation of the immune response in fugu.