Novel hydrogels via click chemistry: Synthesis and potential biomedical applications
Novel hydrogels via click chemistry: Synthesis and potential biomedical applications
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DOI：
10.1021/bm0700800
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发表时间：
2007-06-01
影响因子：
6.2
通讯作者：
Lamanna, Raffaele
中科院分区：
文献类型：
--
作者：
Crescenzi, Vittorio;Cornelio, Lisa;Lamanna, Raffaele
采用了一种新的透明质酸水溶液原位快速化学凝胶化方法。简而言之，已经制备了具有赋予叠氮化物或炔末端官能团的侧链的水溶性多糖衍生物。当后两种类型的衍生物在水溶液中混合在一起时，它们引起1，3-偶极环加成反应，导致在室温下快速凝胶化（在催化量的Cu（I）存在下）。凝胶形成的流变学特征，也可以通过红外光谱定性跟踪。所得的凝胶已被研究的溶胀性能，特别是，NMR光谱特征。分别在联苯胺和阿霉素的水溶液中进行凝胶化过程，多糖网络充当药物储库。阿霉素的释放导致对凝胶交联度的良好可控作用。最后，根据SEM分析，使用酿酒酵母细胞的水性悬浮液形成点击凝胶允许获得支架，其中细胞均匀分布并平滑地粘附到内孔表面。24 h后约60%的包埋细胞表现出增殖活性。如本文详述制备的点击凝胶确实具有许多积极的特征，这些特征使得它们在一定程度上成为药物释放和组织工程操作的选择材料。
A novel procedure for the in situ rapid chemical gelation of aqueous solutions of hyaluronan has been employed. In brief, water-soluble polysaccharide derivatives bearing side chains endowed with either azide or alkyne terminal functionality have been prepared. When the latter two types of derivatives are mixed together in aqueous solution they give rise to a 1,3-dipolar cycloaddition reaction resulting in fast gelation (in the presence of catalytic amounts of Cu(I)) at room temperature. Gel formation has been characterized rheologically and could also be followed qualitatively by means of IR spectroscopy. The resulting gels have been studied in terms of swelling properties and, in particular, NMR spectral features. Carrying out the gelation process in aqueous solutions of benzidamine and doxorubicin, respectively, the polysaccharide networks acted as drug reservoirs. The doxorubicin release resulted in well controllable acting upon the gels degree of cross-linking. Finally, formation of the click-gels using aqueous suspensions of Saccharomices cerevisiae yeast cells allowed the obtainment of scaffolds inside which cells were homogeneously distributed and smoothly adhered to the inner pores surfaces, according to SEM analysis. After 24 h about 60% of the entrapped cells exhibited proliferating activity. Click-gels prepared as detailed herein do have a number of positive features that make them, in perspective, materials of choice for drug release and tissue engineering manipulations.