Structure, organization, and chromosomal mapping of the human neurogranin gene (NRGN)
Structure, organization, and chromosomal mapping of the human neurogranin gene (NRGN)
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DOI：
10.1006/geno.1997.4622
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发表时间：
1997-04-15
期刊：
影响因子：
4.4
通讯作者：
Coloma, A
中科院分区：
文献类型：
--
作者：
deArrieta, CM;Jurado, LP;Coloma, A
在这份报告中，鉴定，结构和染色体定位的人神经颗粒蛋白基因（NRGN）的描述。NRGN是大鼠Ng/RC 3基因的人类同源物，其编码在端脑神经元中表达的脑特异性蛋白。人NRGN基因跨度约12 kb，包含4个外显子和3个内含子。所有剪接受体和供体位点符合经典的AG/GT规则。人神经颗粒素序列预测了一个78个氨基酸的蛋白质，其中5个氨基酸由外显子1编码，其余73个氨基酸由外显子2编码。第三和第四外显子含有非翻译序列。人和大鼠编码序列之间的核酸序列的总体同源性程度为90%，在蛋白质水平上具有96%的同一性和97.5%的相似性。NRGN基因仅在脑中表达为单个1.3-kb成熟mRNA。启动子缺乏TATA和CAAT盒，但显示位于AUG起始密码子上游234个碱基的起始元件的共有序列。5 '侧翼区含有转录因子如Spl、GCF、AP 2和PEA 3的多个推定结合位点。对一组辐射杂种的分析已经导致了NRGN基因在YAC 763 A2（CEPH）中的定位，该基因先前定位在11 q24。该基因座包含在与小鼠9号染色体的保守同线性区域中。(C)北京：科学出版社.
In this report the identification, structure, and chromosomal localization of the human neurogranin gene (NRGN) are described. NRGN is the human homolog of the rat Ng/RC3 gene, which encodes a brain-specific protein expressed in telencephalic neurons. The human NRGN gene spans approximately 12 kb and contains four exons and three introns. All splice acceptor and donor sites conform to the canonical AG/GT rule. Human neurogranin sequence predicts a 78-amino-acid protein with 5 amino acids encoded by exon 1 and the remaining 73 amino acids encoded by exon 2. The third and fourth exons contain untranslated sequences. The overall degree of homology between the human and the rat coding sequences is 90% for the nucleic acid sequence, with 96% identity and 97.5% similarity at the protein level. The NRGN gene is expressed exclusively in brain as a single 1.3-kb mature mRNA. The promoter lacks both TATA and CAAT boxes, but shows a consensus sequence for an initiator element located 234 bases upstream from the AUG initiation codon. The 5'-flanking region contains multiple putative binding sites for transcription factors such as Spl, GCF, AP2, and PEA3. Analysis of a panel of radiation hybrids has led to localization of the NRGN gene in YAC 763A2 (CEPH), previously mapped at 11q24. This locus is contained in a region of conserved synteny with mouse chromosome 9. (C) 1997 Academic Press.