Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.)
Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.)
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DOI：
10.1007/s00251-005-0015-z
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发表时间：
2005-07-01
期刊：
影响因子：
3.2
通讯作者：
Sakai, M
中科院分区：
文献类型：
--
作者：
Savan, R;Aman, A;Sakai, M
在鱼类中，已克隆并鉴定了两种免疫球蛋白重链(IgH)基因，即IgM和IGD。最近，从斑马鱼、虹鲑鱼和河豚中发现了一种新的硬骨鱼特有的IgH同工型。在斑马鱼中，这种新基因的结构域位于Mu区上游，沿着IGH基因座。在本研究中，从鲤鱼中发现了一种新的IgH嵌合体(IgM-IgZ)。该克隆的cDNA编码一个典型的前导肽、一个可变区、两个恒定区和一个分泌尾巴。第一个恒定区由鲤鱼IgM的CH1结构域组成，而第二个恒定区与斑马鱼IgZ的CH4结构域有很高的相似性。分别对L和Zeta结构域进行的Southern杂交研究表明，各自的基因至少存在三个拷贝，Mu和Zeta结构域可能存在于相同的基因座上，尽管相距很远。对IgH基因的表达研究表明，在内毒素刺激下，嵌合免疫球蛋白基因的转录增加。
In fish, two types of immunoglobulin heavy chain (IGH) genes, namely, IgM and IgD, have been cloned and characterized. Recently, a new IGH isotype specific to teleosts had been identified from zebra fish, rainbow trout, and fugu. In zebra fish, the domains of this new gene are present upstream of the mu region along the IGH locus. During this study, a novel IGH chimera (IgM-IgZ) has been discovered from common carp. The cloned cDNA encodes a typical leader peptide, a variable region, two constant regions, and a secretory tail. The first constant region is made up of the CH1 domain of carp IgM, while the second constant region shares a high similarity to the CH4 domain of the IgZ from zebrafish. Southern hybridization studies of the L and domains, conducted separately, revealed the presence of at least three copies of the respective genes, and mu and zeta domains might be present on the same loci, although far apart. Expression studies of the IGH genes suggest that there is an increase in chimeric immunoglobulin gene transcription when stimulated with lipopolysaccharide.