Organization and cellular biology of the perichondrial ossification groove of ranvier: a morphological study in rabbits.
Organization and cellular biology of the perichondrial ossification groove of ranvier: a morphological study in rabbits.
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朗维尔软骨膜骨化沟的组织和细胞生物学：兔子的形态学研究。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
M. Glimcher
中科院分区：
文献类型：
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作者：
F. Shapiro;M. Holtrop;M. Glimcher
本文应用光镜和电镜、~ 3 H-胸腺嘧啶核苷、~ 3 H-脯氨酸和~ 3 H-氨基葡萄糖标记的放射自显影、蛋白多糖和碱性磷酸酶的组织化学染色等方法，对1周龄~ 8月龄家兔的朗氏软骨膜骨化沟进行了研究。通过这些方法，在沟内鉴定出三组细胞：1。沟深处的一组密集的细胞，是成骨细胞的祖细胞，形成骨皮、骨骺生长板区域周围的骨袖套和干骺端的相邻部分。2.一组分布较广、相对未分化的间充质细胞和成纤维细胞，其中一些是可能有助于附着软骨发生和骺软骨宽度生长的成软骨细胞前体。3.在高度定向和组织化的胶原纤维片中的成纤维细胞和纤维细胞，其形成与骨膜的外纤维层和软骨膜连续的纤维层。这一层还将纤维送入骺软骨，并在骨生长进行时将骨膜牢固地锚定在骺上。
The perichondrial ossification groove of Ranvier, a circumferential groove in the periphery of the epiphyseal cartilage, was studied in rabbits whose ages ranged from one week to eight months using light and electron microscopy, autoradiography after labeling with 3H-thymidine, 3H-proline, and 3H-glucosamine, and histochemical staining for proteoglycans and alkaline phosphatase. By these methods, three groups of cells were identified within the groove: 1. A group of densely packed cells deep in the groove, which are the progenitor cells for the osteoblasts that form the bone bark, a cuff of bone surrounding the epiphyseal growth-plate region and the adjacent part of the metaphysis. 2. A group of more widely dispersed, relatively undifferentiated mesenchymal cells and fibroblasts, some of which are chondroblast precursors that probably contribute to appositional chondrogenesis and growth in width of the epiphyseal cartilage. 3. Fibroblasts and fibrocytes among sheets of highly oriented and organized collagen fibers which form a fibrous layer that is continuous with the outer fibrous layer of the periosteum and with the perichondrium. This layer also sends fibers into the epiphyseal cartilage and anchors the periosteum firmly to the epiphyses as bone growth proceeds.