Effects of Partial and Total Isovolemic Exchange Transfusion in Fully Conscious Rats Using Pyridoxylated Polyhemoglobin Solution as a Colloidal Oxygen‐Delivering Blood Replacement Fluid 1
Effects of Partial and Total Isovolemic Exchange Transfusion in Fully Conscious Rats Using Pyridoxylated Polyhemoglobin Solution as a Colloidal Oxygen‐Delivering Blood Replacement Fluid 1
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使用吡啶氧基化多血红蛋白溶液作为胶体氧输送血液替代液对完全清醒大鼠进行部分和全部等容换血的影响 1
DOI：
10.1111/j.1423-0410.1987.tb04905.x
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发表时间：
1987
期刊：
影响因子：
2.7
通讯作者：
T. Chang
中科院分区：
文献类型：
--
作者：
P. Keipert;T. Chang
抽象的。用5′-磷酸吡哆醛（PP）处理的无基质血红蛋白（SFHb）与戊二醛进行分子间交联，得到胶体渗透压= 25 ℃和P50 = 20 ℃的吡哆酰化多聚血红蛋白（PP-PolyHb）溶液（14.0 g/dl）。为了评价PP‐PolyHb作为血液替代品，通过新的连续等容交换输血装置对慢性插管的完全清醒大鼠进行血液置换（30-100%）。测试了结晶SFHb、PP-SFHb、PP-PolyHb、牛血清白蛋白（BSA）和林格氏溶液。与BSA相比，输注PP-PolyHb或PP-SFHb后的呼吸、脉搏和动脉血压更稳定。到交换后7-8天，血液学变化恢复正常。对于主要血液交换，PP-PolyHb的血浆保留时间是结晶SFHb或PP-SFHb的10-15倍。完全置换后，PP‐PolyHb的T1/2为30 h。在置换70%、85%和100%后，长期存活率分别为80%、63%和50%，显著高于（p<0.05）所有其他供试溶液获得的存活率。明确证明了PP-PolyHb优于其他液体。
Abstract. Stroma‐free hemoglobin (SFHb) treated with pyridoxal 5′‐phosphate (PP) was intermolecularly cross‐linked with glutaraldehyde to give pyridoxylated polyhemoglobin (PP‐PolyHb) solution (14.0 g/dl) with colloid osmotic pressure = 25 torr and a P50 = 20 torr. To evaluate PP‐PolyHb as a blood substitute, fully conscious rats with chronic cannulations were subjected to blood replacement (30–100%) by a new continuous isovolemic exchange transfusion set up. Crystalline SFHb, PP‐SFHb, PP‐PolyHb, bovine serum albumin (BSA) and Ringer's solution were tested. Respiration, pulse and arterial blood pressure were more stable following transfusion with PP‐PolyHb or PP‐SFHb compared to BSA. By 7–8 days post‐exchange, hematological changes returned to normal. Plasma retention of PP‐PolyHb was 10–15 times longer than either crystalline SFHb or PP‐SFHb for major blood exchanges. A T 1/2 of 30 h was obtained for PP‐PolyHb after total replacement. Long‐term survival rates of 80, 63 and 50%, following replacement of 70, 85 and 100%, respectively, were significantly higher (p<0.05) than survival obtained for all other solutions tested. Superiority of PP‐PolyHb over other fluids was clearly demonstrated.