Antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro
Antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro
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DOI：
10.1099/jgv.0.001466
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发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Xie, Lilan
中科院分区：
文献类型：
--
作者：
Li, Yaoming;Wang, Jing;Xie, Lilan
新出现的冠状病毒对全世界人类和动物健康构成严重威胁，目前尚无批准的治疗方法。在这里，我们使用传染性胃肠炎病毒（TGEV）作为α冠状病毒模型，并研究了姜黄素对TGEV的抗病毒特性。我们的结果表明，姜黄素以剂量依赖性方式强烈抑制 TGEV 增殖和病毒蛋白表达。我们还观察到姜黄素以剂量、温度和时间依赖性方式表现出直接杀病毒能力。此外，添加时间测定表明姜黄素主要在 TGEV 复制的早期阶段发挥作用。值得注意的是，在吸附测定中，40 mu M的姜黄素导致病毒滴度降低3.55 log TCID50 ml(-1)，表明姜黄素对TGEV的吸附具有优异的抑制作用。总的来说，我们首次证明姜黄素具有杀病毒活性和对 TGEV 的实质抑制作用，表明姜黄素可能是有效控制 TGEV 感染的候选药物。
Emerging coronaviruses represent serious threats to human and animal health worldwide, and no approved therapeutics are currently available. Here, we used Transmissible gastroenteritis virus (TGEV) as the alpha-coronavirus model, and investigated the antiviral properties of curcumin against TGEV. Our results demonstrated that curcumin strongly inhibited TGEV proliferation and viral protein expression in a dose-dependent manner. We also observed that curcumin exhibited direct virucidal abilities in a dose-, temperature- and time-dependent manner. Furthermore, time-of-addition assays showed that curcumin mainly acted in the early phase of TGEV replication. Notably, in an adsorption assay, curcumin at 40 mu M resulted in a reduction in viral titres of 3.55 log TCID50 ml(-1), indicating that curcumin possesses excellent inhibitory effects on the adsorption of TGEV. Collectively, we demonstrate for the first time that curcumin has virucidal activity and virtual inhibition against TGEV, suggesting that curcumin might be a candidate drug for effective control of TGEV infection.