Enzyme-based fiber optic zinc biosensor
Enzyme-based fiber optic zinc biosensor
复制标题
基于酶的光纤锌生物传感器
DOI：
10.1021/ac00054a013
复制
发表时间：
1993
影响因子：
7.4
通讯作者：
E. R. Jones
中科院分区：
文献类型：
--
作者：
R. Thompson;E. R. Jones
描述了一种能够检测纳摩尔浓度的锌（II）的基于荧光的光纤生物传感器。传感器将酶（碳酸酐酶）对离子的特异性识别转换为与活性位点中的锌结合的抑制剂的荧光的变化。金属离子的浓度与两个波长的荧光强度之比成正比，对应于结合和游离抑制剂的发射。锌的感测可以通过单个光纤执行。
A fluoresence-based fiber optic biosensor capable of detecting zinc(II) at nanomolar concentrations is described. The sensor transduces the specific recognition of the ion by an enzyme (carbonic anhydrase) as change in the fluorescence of an inhibitor which binds to the zinc in the active site. The concentration of metal ion is proportional to the ratio of fluorescence intensity at two wavelengths, corresponding to the emission from bound and free inhibitor. Sensing of zinc may be performed through a single optical fiber.