An Allelic Variant of the Broad-Spectrum Blast Resistance Gene Ptr in Weedy Rice Is Associated with Resistance to the Most Virulent Blast Race IB-33
An Allelic Variant of the Broad-Spectrum Blast Resistance Gene Ptr in Weedy Rice Is Associated with Resistance to the Most Virulent Blast Race IB-33
复制标题
杂草稻中广谱稻瘟病抗性基因 Ptr 的等位基因变体与对最强毒稻瘟病种 IB-33 的抗性相关
DOI：
10.1094/pdis-09-21-2043-re
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Jia, Yulin
中科院分区：
文献类型：
--
作者：
Zhao, Haijun;Liu, Yan;Jia, Melissa H.;Jia, Yulin
稻瘟病菌是威胁水稻稳产的重要病原菌之一，水稻抗病基因已被有效地应用于防治稻瘟病。与栽培稻竞争的杂草稻可能携带新的或丢失的R基因。在温室条件下利用杂草-作物作图群体将数量性状位点qBR12.3b定位在黑壳芒（BHA）杂草株系基因组10，633，942-bp和10，820，033-bp的两个单核苷酸多态性标记之间。在这项研究中，我们发现了一部分已知的抗稻瘟病基因Ptren编码的蛋白质与四个犰狳重复序列，并赋予广谱抗稻瘟病。然后，我们分析了来自杂草稻PtrBHA的Ptr基因的序列，并在蛋白质位置874处鉴定了独特的氨基酸谷氨酰胺。通过对PtrBHA的结构分析，预测了PtrBHA基因蛋白质构象的微小变化，表明PtrBHA的产物与抗病性有关。利用PtrBHA基因特异性共显性标记HJ 17 -13，对杂草和作物的等位基因进行了区分。PtrBHA基因存在于同一作图群体的207个个体中，其中qBR12.3b被定位。评估了207名个体及其父母对IB-33的疾病反应。抗性个体有PtrBHA，而敏感个体没有，表明HJ 17 -13对qBR 12.3b的预测是可靠的。总之，这个新开发的标记，和杂草稻基因型携带qBR12.3b，是有用的稻瘟病利用标记辅助选择的改进。
Rice resistance (R) genes have been effectively deployed to prevent blast disease caused by the fungal pathogenMagnaporthe oryzae, one of the most serious threats for stable rice production worldwide. Weedy rice competing with cultivated rice may carry novel or lostRgenes. The quantitative trait locusqBR12.3bwas previously mapped between two single nucleotide polymorphism markers at the 10,633,942-bp and 10,820,033-bp genomic positions in a black-hull-awned (BHA) weed strain using a weed-crop-mapping population under greenhouse conditions. In this study, we found a portion of the known resistance genePtrencoding a protein with four armadillo repeats and confers a broad spectrum of blast resistance. We then analyzed the sequences of thePtrgene from weedy rice,PtrBHA, and identified a unique amino acid glutamine at protein position 874. Minor changes of protein conformation of thePtrBHAgene were predicted through structural analysis ofPtrBHA, suggesting that the product ofPtrBHAis involved in disease resistance. A gene-specific codominant marker HJ17-13 fromPtrBHAwas then developed to distinguish alleles in weeds and crops. ThePtrBHAgene existed in 207 individuals of the same mapping population, whereqBR12.3bwas mapped using this gene-specific marker. Disease reactions of 207 individuals and their parents to IB-33 were evaluated. The resistant individuals hadPtrBHAwhereas the susceptible individuals did not, suggesting that HJ17-13 is reliable to predictqBR12.3b. Taken together, this newly developed marker, and weedy rice genotypes carryingqBR12.3b, are useful for blast improvement using marker assisted selection.