A MUTATION ALLOWING AN MESSENGER-RNA SECONDARY STRUCTURE DIMINISHES TRANSLATION OF SACCHAROMYCES-CEREVISIAE ISO-1-CYTOCHROME-C
A MUTATION ALLOWING AN MESSENGER-RNA SECONDARY STRUCTURE DIMINISHES TRANSLATION OF SACCHAROMYCES-CEREVISIAE ISO-1-CYTOCHROME-C
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DOI：
10.1128/mcb.5.8.1839
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
SHERMAN, F
中科院分区：
文献类型：
--
作者：
BAIM, SB;PIETRAS, DF;SHERMAN, F
酵母S.酿酒酵母在氨基酸位置5和6处产生正常-Ala-Gly-序列的-His-Leu-替换，所述氨基酸位置5和6位于异-1-细胞色素c的内含子区域内;该突变可以适应mRNA中相应位点处发夹结构的形成。改变的蛋白质的量减少到野生型水平的20%，而mRNA的量保持正常。然而，与主要与4-7个核糖体相关的正常CYC 1 + mRNA相反，大部分CYC 1 -239-O mRNA与1-4个核糖体相关。这些结果表明，CYC 1 mRNA翻译区内的稳定二级结构通过抑制延伸减少翻译。
The CYC1-239-O mutation in the yeast S. cerevisiae produces a -His-Leu-replacement of the normal -Ala-Gly-sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in the mRNA. The amount of the altered protein was diminished to 20% of the wild-type level, whereas the amount of the mRNA remained normal. However, in contrast to the normal CYC1+ mRNA that is associated mainly with 4-7 ribosomes, the bulk of the CYC1-239-O mRNA is associated with 1-4 ribosomes. These results suggest that the stable secondary structure within the translated region of the CYC1 mRNA diminishes translation by inhibiting elongation.