Public Availability of a Genotyped Segregating Population May Foster Marker Assisted Breeding (MAB) and Quantitative Trait Loci (QTL) Discovery: An Example Using Strawberry
Public Availability of a Genotyped Segregating Population May Foster Marker Assisted Breeding (MAB) and Quantitative Trait Loci (QTL) Discovery: An Example Using Strawberry
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基因分型分离群体的公开可促进标记辅助育种 (MAB) 和数量性状基因座 (QTL) 发现：以草莓为例
DOI：
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发表时间：
2016
影响因子：
5.6
通讯作者：
C. Weebadde
中科院分区：
文献类型：
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作者：
J. Hancock;S. Sooriyapathirana;N. Bassil;T. Stegmeir;L. Cai;C. Finn;E. van de Weg;C. Weebadde
如果产生一个多样化的分离群体，进行基因分型，并提供给全球育种界，那么与标记辅助育种（MAB）的标记发现相关的大部分成本可以消除。在此，我们提出了一个例子的杂交，野生衍生的家庭的四倍体草莓，可用于其他育种计划，以经济地发现和标记有用的基因的单克隆抗体。利用两个野生种弗吉尼亚草莓和F. chiloensis（FVC 11）的产生和评估的一组表型性状。采用市售全基因组扫描平台（Affytron Axiom IStraw 90 TW），对FVC 11中共计106例个体进行了29，251个单核苷酸多态性（SNP）的基因分型。利用TASSEL软件进行标记性状关联分析。FVC 11群体分离每个母体的女儿、花序数、花序高度、树冠产量、花数、果实大小、产量、内部颜色、可溶性固形物、果实硬度和植物活力。变异系数范围从10%的果实硬度到68%的女儿每母亲，每个性状的基本数量遗传。共发现2,474个SNPs在FVC 11中具有多态性，并且观察到与活力、每母体的女儿数、产量和果实重量有很强的标记性状关联。这些数据表明，FVC 11可用作不同育种计划和地理位置的数量性状基因座检测和后续MAB的参考群体。
Much of the cost associated with marker discovery for marker assisted breeding (MAB) can be eliminated if a diverse, segregating population is generated, genotyped, and made available to the global breeding community. Herein, we present an example of a hybrid, wild-derived family of the octoploid strawberry that can be used by other breeding programs to economically find and tag useful genes for MAB. A pseudo test cross population between two wild species of Fragaria virginiana and F. chiloensis (FVC 11) was generated and evaluated for a set of phenotypic traits. A total of 106 individuals in the FVC 11 were genotyped for 29,251 single nucleotide polymorphisms (SNPs) utilizing a commercially available, genome-wide scanning platform (Affymetrix Axiom IStraw90TW). The marker trait associations were deduced using TASSEL software. The FVC 11 population segregating for daughters per mother, inflorescence number, inflorescence height, crown production, flower number, fruit size, yield, internal color, soluble solids, fruit firmness, and plant vigor. Coefficients of variations ranged from 10% for fruit firmness to 68% for daughters per mother, indicating an underlying quantitative inheritance for each trait. A total of 2,474 SNPs were found to be polymorphic in FVC 11 and strong marker trait associations were observed for vigor, daughters per mother, yield and fruit weight. These data indicate that FVC 11 can be used as a reference population for quantitative trait loci detection and subsequent MAB across different breeding programs and geographical locations.