Paradoxical ATP Elevation in Ischemic Penumbra Revealed by Quantitative Imaging Mass Spectrometry
Paradoxical ATP Elevation in Ischemic Penumbra Revealed by Quantitative Imaging Mass Spectrometry
复制标题
DOI：
10.1089/ars.2010.3290
复制
发表时间：
2010-10-01
影响因子：
6.6
通讯作者：
Suematsu, Makoto
中科院分区：
文献类型：
--
作者：
Hattori, Katsuji;Kajimura, Mayumi;Suematsu, Makoto
脑缺血时能量代谢的局部反应是不均匀的，以致于不能解释缺氧核心区和邻近可挽救区域（如半影区）之间的氧化还原分布。成像质谱结合毛细管电泳/质谱提供定量代谢组学揭示了时空变化的腺苷酸和NADH在小鼠大脑中动脉闭塞模型。与ATP减少的核心不同，尽管血液供应受到限制，但半影区显示ATP的反常升高。值得注意的是，缺血区域中的NADH升高被ATP消耗核心清楚地划分。结果表明，缺血半暗带的代谢并不被动地响应于受损的循环，而是主动地补偿能量费用。抗氧化剂。氧化还原信号。13，1157-1167.
Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass spectrometry providing quantitative metabolomics revealed spatio-temporal changes in adenylates and NADH in a mouse middle-cerebral artery occlusion model. Unlike the core where ATP decreased, the penumbra displayed paradoxical elevation of ATP despite the constrained blood supply. It is noteworthy that the NADH elevation in the ischemic region is clearly demarcated by the ATP-depleting core. Results suggest that metabolism in ischemic penumbra does not respond passively to compromised circulation, but actively compensates energy charges. Antioxid. Redox Signal. 13, 1157-1167.