Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles
Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles
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DOI：
10.1006/bbrc.1997.7913
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发表时间：
1998-01-14
影响因子：
3.1
通讯作者：
Hagiwara, M
中科院分区：
文献类型：
--
作者：
Kuroyanagi, N;Onogi, H;Hagiwara, M
SR蛋白特异性激酶1（SRPK1）是SR剪接因子的特异性激酶。通过RT-PCR的保守激酶结构域，新的SR蛋白特异性激酶克隆从小鼠脑中分离。克隆的cDNA编码106 kDa蛋白质（648个氨基酸，与人SRPK1 92%相同）和120 kDa蛋白质（681个氨基酸，与人SRPK1 58%相同）。因此，将它们分别命名为mSRPK1和mSRPK2。北方印迹显示mSRPK 1在所有检测的组织中普遍表达，mSRPK 2在睾丸、肺和脑中具有组织特异性表达。这两种激酶在体外磷酸化SR蛋白的成员SF 2/ASF，并且磷酸肽图谱是相同的，表明这两种激酶磷酸化SF 2/ASF的同一位点。过表达mSRPK2导致共转染的SF 2/ASF和内源性SC 35解体。我们的研究结果表明，SRPK家族成员可能会调节SR蛋白的解体，在组织特异性的方式。(C)北京：科学出版社.
SR-protein-specific kinase 1 (SRPK1) is first identified as a specific kinase for SR splicing factors. By RT-PCR of a conserved kinase domain, novel SR-protein-specific kinase clones were isolated from mouse brain. The cloned cDNAs encode a 106 kDa protein (648 amino acids, 92% identical to human SRPK1) and a 120 kDa protein (681 amino acids, 58% identical to human SRPK1). Therefore, they were designated mSRPK1 and mSRPK2, respectively. Northern blotting revealed the ubiquitous expression of mSRPK1 in all tissues examined and the tissue-specific expression of mSRPK2 in testis, lung, and brain. Both kinases phosphorylated SF2/ASF, a member of SR proteins in vitro and the phosphopeptide mappings were identical, indicating that these kinases phosphorylate the same site of SF2/ ASF. Overexpression of mSRPK2 caused disassembly of cotransfected SF2/ASF and endogenous SC35. Our results indicate that SRPK family members may regulate the disassembly of the SR proteins in a tissue-specific manner. (C) 1998 Academic Press.