Global expression of cell surface proteins in embryonic stem cells.
Global expression of cell surface proteins in embryonic stem cells.
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胚胎干细胞中细胞表面蛋白的整体表达
DOI：
10.1371/journal.pone.0015795
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发表时间：
2010-12-29
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Gu B;Zhang J;Wang W;Mo L;Zhou Y;Chen L;Liu Y;Zhang M
最近的研究表明，胚胎干（ES）细胞在mRNA水平上表达基因组中的大多数基因；然而，目前尚不清楚这种全局表达是否传播到蛋白质水平。细胞表面蛋白可以在胚胎干细胞中发挥关键功能，因此确定胚胎干细胞是否全局表达细胞表面蛋白将对胚胎干细胞生物学具有重要意义。采用生物素标记法纯化小鼠胚胎干细胞表面蛋白，并进行蛋白质组学分析。鉴定出约1000种跨膜或分泌的细胞表面蛋白。这些蛋白质涵盖了多种功能类别，包括信号转导，粘附和运输。此外，mES细胞混杂表达多种组织特异性表面蛋白。许多表面蛋白在mES细胞上异质表达。我们还发现人类胚胎干细胞表达多种组织特异性表面蛋白。我们的研究结果表明，全局基因表达不仅仅是基因表达泄漏的结果，这可能归因于ES细胞松散的染色质结构；它还被传播到职能层。胚胎干细胞可能使用不同的表面蛋白来接收来自不同细胞外刺激的信号，从而启动分化。此外，组织特异性表面蛋白的混杂表达为细胞表面标记筛选策略提供了新的见解。
Recent studies have shown that embryonic stem (ES) cells globally express most genes in the genome at the mRNA level; however, it is unclear whether this global expression is propagated to the protein level. Cell surface proteins could perform critical functions in ES cells, so determining whether ES cells globally express cell surface proteins would have significant implications for ES cell biology. The surface proteins of mouse ES cells were purified by biotin labeling and subjected to proteomics analysis. About 1000 transmembrane or secreted cell surface proteins were identified. These proteins covered a large variety if functional categories including signal transduction, adhesion and transporting. More over, mES cells promiscuously expressed a wide variety of tissue specific surface proteins. And many surface proteins were expressed heterogeneously on mES cells. We also find that human ES cells express a wide variety of tissue specific surface proteins. Our results indicate that global gene expression is not simply a result of leaky gene expression, which could be attributed to the loose chromatin structure of ES cells; it is also propagated to the functional level. ES cells may use diverse surface proteins to receive signals from the diverse extracellular stimuli that initiate differentiation. Moreover, the promiscuous expression of tissue specific surface proteins illuminate new insights into the strategies of cell surface marker screening.
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