IDENTIFICATION OF 2 NUCLEAR GENES (ATP11, ATP12) REQUIRED FOR ASSEMBLY OF THE YEAST F1-ATPASE
IDENTIFICATION OF 2 NUCLEAR GENES (ATP11, ATP12) REQUIRED FOR ASSEMBLY OF THE YEAST F1-ATPASE
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DOI：
10.1073/pnas.87.13.4986
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发表时间：
1990-07-01
影响因子：
11.1
通讯作者：
TZAGOLOFF, A
中科院分区：
文献类型：
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作者：
ACKERMAN, SH;TZAGOLOFF, A
已经筛选了酿酒酵母的核分裂缺陷突变体（pet突变体）的线粒体ATP酶缺陷。发现两个互补组中的突变体具有野生型ATP酶活性的10%或更少。由突变体定义的两个野生型核基因被命名为ATP 11和ATP 12。由这两个基因编码的蛋白质不是ATP酶的亚基，而是在F1合成的后期阶段，在将亚基运输到线粒体的内部隔室中之后，似乎行使重要的功能。atp 11和atp 12突变体的线粒体仅具有略微降低的α-ATP酶水平。和βF1的亚基。这两种蛋白质都被加工成成熟的大小，但不是天然F1结构的一部分，也不是与线粒体膜相关的。对突变表型最合理的解释是F1寡聚体组装受阻。
Nuclear respiratory-deficient mutants of Saccharomyces cerevisiae (pet mutants) have been screened for defects in the mitochondrial ATPase. Mutants in two complementation groups were found to have 10% or less of wild-type ATPase activity. The two wild-type nuclear genes defined by the mutants have been designated ATP11 and ATP12. The proteins encoded by the two genes are not subunits of the ATPase but rather appear to exercise an important function at a late stage in the synthesis of F1 after transport of the subunits into the internal compartment of mitochondria. Mitochondria of atp11 and atp12 mutants have only marginally reduced levels of the .alpha. and .beta. subunits of F1. Both proteins are processed to their mature size but are not part of a native F1 structure or associated with the mitochondrial membrane. The most reasonable explanation for the mutant phenotype is a block in the assembly of the F1 oligomer.