Comparison of measuring energy metabolism by different 31P‐magnetic resonance spectroscopy techniques in resting, ischemic, and exercising muscle
Comparison of measuring energy metabolism by different 31P‐magnetic resonance spectroscopy techniques in resting, ischemic, and exercising muscle
复制标题
不同 31P 磁共振波谱技术测量静息、缺血和运动肌肉能量代谢的比较
DOI：
--
复制
发表时间：
2012
影响因子：
3.3
通讯作者：
M. Roden
中科院分区：
文献类型：
--
作者：
A. I. Schmid;V. Schrauwen;M. Andreas;M. Wolzt;E. Moser;M. Roden
另一种量化线粒体活性或功能的方法被广泛用于研究胰岛素抵抗和2型糖尿病，即饱和转移和磷酸肌酸(PCR)恢复。由于这些方法实际上是确定不同的参数，本研究旨在比较饱和转移结果与同一组内的PCR回收率测量结果。15名受试者进行了饱和转移和缺血运动恢复实验。由袖带扩张引起的缺血(Q)时，PCR值的降低是静息三磷酸腺苷(ATP)产生的另一项指标。饱和转移法测得的三磷酸腺苷合成率(0.234±0.043 mm/S)大于(Q=0.0077±0.0011 mm/S)，但与Q呈正相关(r=0.63P=0.013)。回归参数与fATP值(Qmax，Lin：r=0.71，P=0.003，Qmax，ADP：r=0.66，P=0.007)和Q(Qmax，Lin：r=0.92，P=0.000002，Qmax，ADP：r=0.76，P=0.001)具有良好的相关性。综上所述，尽管在缺血时饱和转移产生的ATP合成率高于PCR值的降低，但两者之间的显著相关性表明fATP可以作为线粒体活性的标志。Q和fATP都与PCR恢复动力学相关的发现表明，骨骼肌具有更高的最大有氧ATP合成速率，在静止时代谢也更活跃。Magn Reson Med，2011。©2011 Wiley-Liss，Inc.
Alternate methods to quantify mitochondrial activity or function have been extensively used for studying insulin resistance and type 2 diabetes mellitus, namely saturation transfer and phosphocreatine (PCr) recovery. As these methods are in fact determining different parameters, this study aimed to compare saturation transfer results to PCr recovery measurements within the same group. Fifteen subjects underwent saturation transfer and ischemic exercise‐recovery experiments. PCr decrease during ischemia (Q), induced by cuff inflation, served as an additional measure of resting ATP (adenosine triphosphate) production. ATP synthetic rate (fATP) measured by saturation transfer (0.234 ± 0.043 mM/s) was greater than (Q = 0.0077 ± 0.0011 mM/s), but correlated well with Q (r = 0.63 P = 0.013). Parameters of PCr recovery correlated well with fATP (Qmax,lin: r = 0.71, P = 0.003, Qmax,ADP: r = 0.66, P = 0.007) and Q (Qmax,lin: r = 0.92, P = 0.000002, Qmax,ADP: r = 0.76, P = 0.001). In conclusion, although saturation transfer yields higher ATP synthetic rates than PCr decrease during ischemia, their significant correlation indicates that fATP can be used as a marker of mitochondrial activity. The finding that both Q and fATP correlate with PCr recovery kinetics suggests that skeletal muscle with greater maximal aerobic ATP synthetic rates is also metabolically more active at rest. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
影响因子：
7.7
作者：
Kelley, DE;He, J;Ritov, VB
通讯作者：
Ritov, VB
影响因子：
158.5
作者：
Petersen, KF;Dufour, S;Shulman, GI
通讯作者：
Shulman, GI