Optical method for the determination of the oxygen-transfer capacity of small bioreactors based on sulfite oxidation
Optical method for the determination of the oxygen-transfer capacity of small bioreactors based on sulfite oxidation
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DOI：
10.1002/bit.1126
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发表时间：
2001-09-05
影响因子：
3.8
通讯作者：
Büchs, J
中科院分区：
文献类型：
--
作者：
Hermann, R;Walther, N;Büchs, J
微生物的生长可能受到氧气供应不足的操作条件的限制。为了确定小型生物反应器（如摇瓶、试管和微量滴定板）的氧传递能力，开发了一种基于亚硫酸盐氧化的非侵入性易用光学方法。亚硫酸钠的模型系统，首先在摇瓶实验中优化这一特殊应用。调节反应条件（pH、缓冲液和催化剂浓度）以在整个亚硫酸盐氧化反应期间获得恒定的氧转移速率。在氧化结束时pH的急剧下降（这对于该反应是典型的）通过加入pH染料来可视化，并用于测量反应时间的长度。然后可以通过在亚硫酸钠的完全化学计量转化期间消耗的氧和视觉确定的反应时间来计算氧转移能力。该光学测量方法用于测定小规模生物反应器中的氧传递能力的适用性通过应用氧电极的独立物理方法得到证实。在摇瓶中测定了化学模型系统与恶臭假单胞菌CA-3培养物之间最大氧传递能力的相关因子。最后，将新开发的光学测量方法用于不同类型的透明小型生物反应器的氧传递能力的测定。(C)John Wiley & Sons，Inc.
The growth of microorganisms may be limited by operating conditions which provide an inadequate supply of oxygen. To determine the oxygen-transfer capacities of small-scale bioreactors such as shaking flasks, test tubes, and microtiter plates, a noninvasive easy-to-use optical method based on sulfite oxidation has been developed. The model system of sodium sulfite was first optimized in shaking-flask experiments for this special application. The reaction conditions (pH, buffer, and catalyst concentration) were adjusted to obtain a constant oxygen transfer rate for the whole period of the sulfite oxidation reaction. The sharp decrease of the pH at the end of the oxidation, which is typical for this reaction, is visualized by adding a pH dye and used to measure the length of the reaction period. The oxygen-transfer capacity can then be calculated by the oxygen consumed during the complete stoichiometric transformation of sodium sulfite and the visually determined reaction time. The suitability of this optical measuring method for the determination of oxygen-transfer capacities in small-scale bioreactors was confirmed with an independent physical method applying an oxygen electrode. The correlation factor for the maximum oxygen-transfer capacity between the chemical model system and a culture of Pseudomonas putida CA-3 was determined in shaking flasks. The newly developed optical measuring method was finally used for the determination of oxygen-transfer capacities of different types of transparent small-scale bioreactors. (C) 2001 John Wiley & Sons, Inc.