Silk fibroin/poly-(L-lactide-co-caprolactone) nanofiber scaffolds loaded with Huangbai Liniment to accelerate diabetic wound healing
Silk fibroin/poly-(L-lactide-co-caprolactone) nanofiber scaffolds loaded with Huangbai Liniment to accelerate diabetic wound healing
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丝素蛋白/聚(L-丙交酯-己内酯)纳米纤维支架负载黄柏搽剂加速糖尿病伤口愈合
DOI：
10.1016/j.colsurfb.2021.111557
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发表时间：
2021-01-09
影响因子：
5.8
通讯作者：
Mo, Xiumei
中科院分区：
文献类型：
--
作者：
Xu, Xiaoqing;Wang, Xiangsheng;Mo, Xiumei
糖尿病感染是一种难以治愈的长期并发症。糖尿病患者的皮肤容易损伤，损伤后愈合缓慢，伤口反复发生。因此，迫切需要开发一种治疗糖尿病伤口的有效方法。本研究采用静电纺丝技术将黄柏搽剂（复方黄柏液，CPL）负载于蚕丝蛋白（SF）/聚（L-丙交酯-己内酯）（PLCL）中，制备治疗糖尿病创面的黄柏膜（SP/CPL）。用扫描电子显微镜（SEM）观察了纳米纤维的形貌和结构。扫描电镜结果表明，随着药物浓度的增加，纤维表面光滑无珠，纤维直径减小。释放曲线表明药物的持续释放。此外，载药纳米纤维显示出对金黄色葡萄球菌和大肠杆菌的抑制作用。此外，体外细胞培养研究显示NIH-3 T3细胞在含药生物膜上的增殖和粘附增加。动物实验表明，负载CPL的生物膜在伤口愈合过程中增加了TGF-β信号通路和胶原蛋白的表达，抑制了促炎因子的表达，从而有效促进糖尿病小鼠的伤口愈合。因此，具有CPL负载的SP/CPL可降解支架是糖尿病伤口敷料和组织工程的潜在候选者。
Diabetic infection is a long-term complication difficult to cure. The skin of diabetic patients is prone to damage, the healing is slow after the injury, and the wound occurs repeatedly. Therefore, there is an urgent need to develop an effective method for treating diabetes wounds. In this study, we used the electrospinning technique to load Huangbai Liniment (Compound Phellodendmn Liquid, CPL) into Silk fibmin (SF) /poly-(L-lactide-co-caprolactone) (PLCL) to prepare the nanofiber membrane (SP/CPL) to treat the diabetic wound. The morphology and structure of the nanofibers were observed by scanning electron microscope (SEM). The SEM results indicate the smooth and bead free fibers and the diameter of the fiber decreased with increasing drug concentration. The release profile indicates the sustained release of the drug. Moreover, the drug-loaded nanofibers showed inhibitory effects for S.aureus and E.coli. Furthermore, in vitro cell culture studies showed the increased proliferation and adhesion of NIH-3T3 cells on the drug-containing nanofiber membrane. Animal experiments showed that the nanofiber membrane loaded with CPL increases the expression of the TGF-beta signaling pathway and collagen during wound healing, inhibits the expression of pro-inflammatory factors, and thus effectively promotes wound healing in diabetic mice. Therefore, the SP/CPL nanofiber scaffold with CPL loading is a potential candidate for diabetic wound dressings and tissue engineering.