Proteomic Response and Quality Maintenance in Postharvest Fruit of Strawberry (Fragaria × ananassa) to Exogenous Cytokinin.
Proteomic Response and Quality Maintenance in Postharvest Fruit of Strawberry (Fragaria × ananassa) to Exogenous Cytokinin.
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草莓（Fragaria x ananassa）采后果实对外源细胞分裂素的蛋白质组响应和质量维持
DOI：
10.1038/srep27094
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发表时间：
2016-06-01
影响因子：
4.6
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Li L;Li D;Luo Z;Huang X;Li X
目前对水果对植物生长调节剂应用的反应分子机制的理解存在局限性，这已日益成为提高作物品质的主要挑战。本研究旨在评估草莓对采前施用外源细胞分裂素氯吡脲（CPPU）的反应。在不同条件下储存后，对采后内部和生理品质特性进行了表征。通过无标记蛋白质组定量方法进行层次聚类分析，在所有处理中总共鉴定出草莓中的 124 种蛋白质。蛋白质和基因的表达谱涵盖了细胞分裂素在初级和次级代谢、应激反应等中的广泛作用。八十八种蛋白质和五十六种蛋白质分别在收获时和储存后立即受到显着调节。一般来说，草莓在贮藏前的糖酵解仅受CPPU的调节；除了加速光合作用和酸代谢外，与对照相比，施用CPPU还使草莓在贮藏后保持了较高的抵抗胁迫刺激的能力。然而，草莓中的挥发性生物合成已被外源CPPU抑制。除了主要转录组表达之外，还鉴定了新的细胞分裂素反应蛋白和过程，以深入了解植物激素对水果采后质量的控制。
The limitations in current understanding of the molecular mechanisms underlying fruit response to the application of plant growth regulators have increasingly become major challenges in improvement of crop quality. This study aimed to evaluate the response of strawberry to the preharvest application of exogenous cytokinin known as forchlorfenuron (CPPU). Postharvest internal and physiological quality attributes were characterized following storage under different conditions. Hierarchical clustering analysis via a label-free proteomic quantitative approach identified a total of 124 proteins in strawberries across all treatments. The expression profiles of both proteins and genes spanned the ranged role of cytokinin involved in primary and secondary metabolism, stress response, and so on. Eighty-eight proteins and fifty-six proteins were significantly regulated immediately at harvest and after storage, respectively. In general, the glycolysis in strawberry was only regulated by CPPU before storage; in addition to the accelerated photosynthesis and acid metabolism, CPPU application maintained higher capacity of resistance in strawberry to stress stimuli after storage, in comparison to control. Nevertheless, the volatile biosynthesis in strawberry has been suppressed by exogenous CPPU. Novel cytokinin response proteins and processes were identified in addition to the main transcriptomic expression to gain insights into the phytohormone control of fruit postharvest quality.