Quantitative secretome analysis of polymyxin B resistance in Escherichia coli
Quantitative secretome analysis of polymyxin B resistance in Escherichia coli
复制标题
大肠杆菌多粘菌素 B 耐药性的定量分泌组分析
DOI：
10.1016/j.bbrc.2020.07.010
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
Sun Xuesong
中科院分区：
文献类型：
--
作者：
Yang Dong-Hong;Liu Shiqin;Cao Linlin;Zheng Yun-Dan;Huang Jian-Fang;Ge Ruiguang;He Qing-Yu;Sun Xuesong
细菌耐药性已成为人类健康的严重威胁。特别是，对人类感染的最后一道防线多粘菌素的耐药性的逐渐发展是一个主要问题。分泌的蛋白质有助于细菌与环境之间的相互作用。在这项研究中，我们比较了多粘菌素B敏感和耐药的大肠杆菌菌株的分泌组的数据独立采集质谱。共鉴定出87个多粘菌素B耐药大肠埃希菌与敏感菌株分泌蛋白的差异表达。GO富集分析表明，差异表达的蛋白质参与了细菌型鞭毛依赖的细胞运动、离子转运、碳水化合物衍生物生物合成过程、细胞对刺激的反应、细胞器组织和细胞壁组织或生物发生等生物学过程。多粘菌素B耐药菌分泌蛋白的差异表达富集了多种途径，表明耐药表型依赖于复杂的调控机制。在多粘菌素B耐药大肠杆菌中发现了一种潜在的生物标志物或药物靶点（YebV）。这项工作阐明了与获得多粘菌素耐药性相关的分泌组变化，并可能有助于药物开发。
Bacterial resistance has become a serious threat to human health. In particular, the gradual development of resistance to polymyxins, the last line of defense for human infections, is a major issue. Secreted proteins contribute to the interactions between bacteria and the environment. In this study, we compared the secretomes of polymyxin B-sensitive and -resistantEscherichia colistrains by data-independent acquisition mass spectrometry. In total, 87 differentially expressed secreted proteins were identified in polymyxin B-resistantE. colicompared to the sensitive strain. A GO enrichment analysis indicated that the differentially expressed proteins were involved in biological processes, including bacterial-type flagellum-dependent cell motility, ion transport, carbohydrate derivative biosynthetic process, cellular response to stimulus, organelle organization, and cell wall organization or biogenesis. The differentially expressed secreted proteins in polymyxin B-resistant bacteria were enriched for multiple pathways, suggesting that the resistance phenotype depends on complex regulatory mechanisms. A potential biomarker or drug target (YebV) was found in polymyxin B-resistantE. coli. This work clarifies the secretome changes associated with the acquisition of polymyxin resistance and may contribute to drug development.