99mTc-Hynic-annexin V imaging to evaluate inflammation and apoptosis in rats with autoimmune myocarditis
99mTc-Hynic-annexin V imaging to evaluate inflammation and apoptosis in rats with autoimmune myocarditis
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DOI：
10.1007/s00259-002-1006-z
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发表时间：
2003-02-01
影响因子：
9.1
通讯作者：
Nishimura, T
中科院分区：
文献类型：
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作者：
Tokita, N;Hasegawa, S;Nishimura, T
炎症和细胞死亡是心肌炎的两个重要组成部分。我们使用两种放射性示踪剂-锝-99m-膜联蛋白V （tc -99m-膜联蛋白）作为凋亡细胞死亡的标记物和碳-14脱氧葡萄糖（C-14-DG）作为炎症的标记物，评估了自身免疫性心肌炎大鼠炎症和凋亡细胞死亡的分布，并与组织学结果进行了比较。接种猪心肌蛋白3周、7周和14周后，分别注射Tc-99m-annexin和C-14-DG（急性期、亚急性期和慢慢性）。通过组织计数，以每克注射剂量的百分比（% ID/g）来确定全心脏的摄取。采用Tc-99m-annexin和C-14-DG进行双示踪放射自显影。将这些药物的分布与苏木精和伊红染色的结果进行比较，以确定炎症区域，TUNEL染色确定凋亡区域。心肌炎急性期心肌总摄取tc -99m-膜联蛋白显著高于正常大鼠(1.28% 0.30% vs 0.46%+/-0.01%
Inflammation and cell death are two important components of myocarditis. We evaluated the distribution of inflammation and apoptotic cell death in rats with autoimmune myocarditis using two radiotracers - technetium-99m Hynic-annexin V (Tc-99m-annexin) as a marker of apoptotic cell death and carbon-14 deoxyglucose (C-14-DG) as a marker of inflammation - in comparison with histologic findings. Three, 7 and 14 weeks after immunization with porcine cardiac myosin (acute, subacute, and chronic phases, respectively) Tc-99m-annexin and C-14-DG were injected. The uptake in the total heart was determined as the percentage of injected dose per gram (% ID/g) by tissue counting. Dual-tracer autoradiography with Tc-99m-annexin and C-14-DG was performed. The distribution of each of these agents was compared with the results of hematoxylin and eosin staining to identify areas of inflammation, and TUNEL staining to identify areas of apoptosis. Total cardiac uptake of Tc-99m-annexin in the acute phase of myocarditis was significantly higher than that in normal rats (1.28% 0.30% vs 0.46%+/-0.01%; P