How to change the aggregation in the DNA/surfactant/cationic conjugated polyelectrolyte system through the order of component addition: anionic versus neutral surfactants.
How to change the aggregation in the DNA/surfactant/cationic conjugated polyelectrolyte system through the order of component addition: anionic versus neutral surfactants.
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如何通过组分添加顺序改变 DNA/表面活性剂/阳离子共轭聚电解质系统中的聚集：阴离子表面活性剂与中性表面活性剂。
DOI：
10.1021/la1011764
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. J. Tapia
中科院分区：
文献类型：
--
作者：
M. Monteserín;H. Burrows;R. Mallavia;R. D. Di Paolo;A. Maçanita;M. J. Tapia
研究了双链DNA（dsDNA）、阳离子共轭双链DNA（CPE）、聚[9，9-bis]（6-N，N，N-三甲铵）己基）-芴-亚苯基）]溴化物（HTMA-PFP）和阴离子或中性表面活性剂（十二烷基磺酸钠，SDSu，和正十二烷基五氧杂环己二醇醚，C（12）E（5））在4%（v/v）二甲基亚砜（DMSO）-水中的溶液，使用UV/可见光吸收和荧光光谱法。戏剧性的变化被观察到的光谱行为的系统取决于添加试剂的顺序，表面活性剂的电荷，和浓度范围。如果将中性C（12）E（5）加入HTMA-PFP/dsDNA复合物中，则未观察到显著的光谱变化。然而，如果SDSu被添加到相同的复合物，一个显着增加的吸光度和发射强度观察表面活性剂浓度高于临界胶束浓度（CMC）。相比之下，如果将dsDNA加入HTMA-PFP/表面活性剂体系（表面活性剂浓度高于其cmc）中，则SDSu没有观察到显著变化，而C（12）E（5）观察到聚合物发射的急剧淬灭，这可以根据HTMA-PFP/表面活性剂/DNA络合和随后的聚合物在电荷中和时聚集来定量解释。结果进行了比较，与那些二元系统（HTMA-PFP/DNA和HTMA-PFP/表面活性剂），并表明静电相互作用的重要性HTMA-PFP和相反电荷的物种在聚集过程中。
The competitive interaction has been studied between double-stranded DNA (dsDNA), the cationic conjugated polyelectrolyte (CPE) poly[9,9-bis(6-N,N,N-trimethylamonium)hexyl)-fluorene-phenylene)] bromide (HTMA-PFP) and anionic or neutral surfactants (sodium dodecyl sulfonate, SDSu, and n-dodecyl pentaoxyethylene glycol ether, C(12)E(5)) in 4% (v/v) dimethyl sulfoxide (DMSO)-water using UV/visible absorption and fluorescence spectroscopy. Dramatic changes are observed in the spectroscopic behavior of the system depending on the order of addition of the reagents, the surfactant charge, and concentration range. If the neutral C(12)E(5) is added to the HTMA-PFP/dsDNA complex, no significant spectroscopic changes are observed. However, if SDSu is added to the same complex, a dramatic increase of the absorbance and emission intensity is observed for surfactant concentrations above the critical micelle concentration (cmc). In contrast, if dsDNA is added to HTMA-PFP/surfactant systems (with surfactant concentrations above their cmc) no significant changes are observed with SDSu, while a dramatic quenching of polymer emission is observed with C(12)E(5), which can be explained quantitatively in terms of HTMA-PFP/surfactant/DNA complexation and the subsequent polymer aggregation upon charge neutralization. The results are compared with those for the binary systems (HTMA-PFP/DNA and HTMA-PFP/surfactants) and indicate the importance of electrostatic interactions between HTMA-PFP and oppositely charged species in the aggregation processes.