A flea-associated rickettsia pathogenic for humans
A flea-associated rickettsia pathogenic for humans
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DOI：
10.3201/eid0701.010112
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发表时间：
2001-01-01
影响因子：
11.8
通讯作者：
de Lamballerie, X
中科院分区：
文献类型：
--
作者：
Raoult, D;La Scola, B;de Lamballerie, X
1990年，通过分子生物学技术在美国跳蚤中发现了一种名为ELB因子或“猫立克次体”的立克次体，后来又在德克萨斯州和墨西哥的四名患者中发现了这种立克次体。我们试图在不同的温度和条件下从受感染的跳蚤中分离出这种立克次体。对ELB病原体的代表性分离株马赛菌株进行了表征，并用于开发检测人血清中反应性抗体的微量免疫荧光试验。从20组经聚合酶链反应证实的感染跳蚤中的19组中分离出ELB因子。微量免疫荧光结果提供了法国（2）和巴西（2）4例发热和皮疹患者感染ELB因子的血清学证据，支持这种立克次体的致病作用。我们成功分离出这种立克次体，使其可用于血清学检测，以确定其临床谱、患病率和分布。
A rickettsia named the ELB agent, or "Rickettsia felis," was identified by molecular biology techniques in American fleas in 1990 and later in four patients from Texas and Mexico. We attempted to isolate this rickettsia from infected fleas at various temperatures and conditions. A representative isolate of the ELB agent, the Marseille strain, was characterized and used to develop a microimmunofluorescence test that detected reactive antibodies in human sera. The ELB agent was isolated from 19 of 20 groups of polymerase chain reaction-proven infected fleas. The microimmunofluorescence results provided serologic evidence of infection by the ELB agent in four patients with fever and rash in France (2) and Brazil (2), supporting the pathogenic role of this rickettsia. Our successful isolation of this rickettsia makes it available for use in serologic tests to determine its clinical spectrum, prevalence, and distribution.