The Community Effect and Purkinje Cell Migration in the Cerebellar Cortex: Analysis of Scrambler Chimeric Mice
The Community Effect and Purkinje Cell Migration in the Cerebellar Cortex: Analysis of Scrambler Chimeric Mice
复制标题
小脑皮质中的群落效应和浦肯野细胞迁移：扰码嵌合小鼠的分析
DOI：
10.1523/jneurosci.22-02-00464.2002
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Goldowitz
中科院分区：
文献类型：
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作者：
Huaitao Yang;Patricia Jensen;D. Goldowitz
已知小鼠中的Disabled-1蛋白是Reelin分子通路的细胞间信号传导组分，其在脑和脊髓中的几种结构中促进神经元迁移。scrambler突变小鼠与reeler小鼠在表型上相同，是由于disabled-1基因的突变（豪厄尔等人，1997;谢尔顿等人，1997年）。小脑的浦肯野细胞表达Disabled-1，并且在扰码突变小鼠中经历大规模的迁移失败（豪厄尔等人，1997;谢尔顿等人，1997; Gallagher等人，1998; Rice等人，1998年）。我们试图通过研究浦肯野细胞群在实验小鼠聚集嵌合体使用的细胞标志物，允许识别来自突变谱系的神经元，以确定这种突变的发展基础。我们发现，一个遗传上正常的组成部分，环境不能帮助扰频突变浦肯野细胞在迁移过程中。然而，环境中突变组分的存在可导致野生型浦肯野细胞异位。似乎有一个线性关系之间的百分比小脑是遗传突变和野生型浦肯野细胞表达突变表型的数量。这些研究指出，在中枢神经系统发育过程中，神经元迁移形成层状结构的过程中，细胞内在和细胞外在特性之间存在相互作用。
The Disabled-1 protein in mouse is known to be an intercellular signaling component of the Reelin molecular pathway that subserves neuronal migration in several structures in the brain and spinal cord. The scrambler mutant mouse, which is phenotypically identical to the reeler mouse, is due to a mutation in the disabled-1gene (Howell et al., 1997; Sheldon et al., 1997). The Purkinje cells of the cerebellum express Disabled-1 and experience a massive failure of migration in the scrambler mutant mouse (Howell et al., 1997; Sheldon et al., 1997; Gallagher et al., 1998; Rice et al., 1998). We sought to define the developmental basis of this mutation by studying the Purkinje cell population in experimental mouse aggregation chimeras using a cell marker that permitted the identification of neurons derived from the mutant lineage. We found that a genetically normal component to the environment cannot assist scrambler mutant Purkinje cells in the migratory process. However, the presence of a mutant component to the environment can cause the ectopia of wild-type Purkinje cells. There appears to be a linear relationship between the percentage of the cerebellum that is genetically mutant and the number of wild-type Purkinje cells that express a mutant phenotype. These studies point to the interplay between cell-intrinsic and cell-extrinsic properties in the migration of neurons to form laminated structures during CNS development.
影响因子：
4.6
作者：
D. Rice;M. Sheldon;G. D’Arcangelo;K. Nakajima;D. Goldowitz;T. Curran
通讯作者：
D. Rice;M. Sheldon;G. D’Arcangelo;K. Nakajima;D. Goldowitz;T. Curran
重新审视小脑突变小鼠和嵌合体。
DOI：
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发表时间：
1997
期刊：
Perspectives on developmental neurobiology.
影响因子：
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作者：
Mullen,RJ;Hamre,KM;Goldowitz,D
通讯作者：
Goldowitz,D
曲尾对颗粒细胞前体的固有作用破坏小脑发育：对曲尾嵌合小鼠的分析。
DOI：
10.1242/dev.124.21.4201
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hamre,KM;Goldowitz,D
通讯作者：
Goldowitz,D