Characterization of the exosporium of Bacillus cereus
Characterization of the exosporium of Bacillus cereus
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DOI：
10.1046/j.1365-2672.1999.00878.x
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发表时间：
1999-08-01
影响因子：
4
通讯作者：
Moir, A
中科院分区：
文献类型：
--
作者：
Charlton, S;Moir, AJG;Moir, A
通过凝胶电泳纯化并分离来自 Bacillus rereus ATCC 10876 内孢子的外孢子成分。一些已恢复 N 末端序列的蛋白质被发现在蛋白质数据库中具有同源物，这些同源物已被证明在其他生物体中具有酶活性。其中包括锌金属蛋白酶、免疫抑制剂 A，已在苏云金芽孢杆菌中进行了描述。它已被证明以活性 73 kDa 形式存在于蜡样芽孢杆菌的外孢子上。与外孢子相关的其他蛋白质包括分子伴侣 GroEL 和枯草芽孢杆菌的 RocA（1-吡咯啉-5-羧酸脱氢酶（EC 1.5，1.12））的同源物。尽管这些不太可能代表孢子外膜的完整结构蛋白，但观察到它们选择性地存在于孢子表面层表明该层可能具有功能意义。
Exosporium components from endospores of Bacillus rer eus ATCC 10876 were purified and separated by gel electrophoresis. Several of the proteins for which N-terminal sequences were recovered were found to have homologues in protein databases which have been demonstrated to have enzymic activity in other organisms. Amongst these is a zinc metalloprotease, immune inhibitor A, already described in B. thuringiensis. This has been shown to be present in an active 73 kDa form on the exosporium of B. cereus. Other proteins associated with the exosporium include the molecular chaperone GroEL and a homologue of RocA (1-pyrroline-5-carboxylate dehydrogenase (EC 1.5, 1.12)) of B, subtilis. Although these are unlikely to represent integral structural proteins of the exosporium, the observation that they are selectively present in the spore surface layer suggests that this layer may have functional significance.