Influence of tissue and geographic locality on culturable endophytic bacteria of Atractylodes macrocephala.
Influence of tissue and geographic locality on culturable endophytic bacteria of Atractylodes macrocephala.
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DOI：
10.1099/mic.0.001109
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发表时间：
2021-11
期刊：
影响因子：
1.5
通讯作者：
Wei Wu;Shiyu Wang;Jianjun Wu;B. He;B. Zhu;L. Qin
中科院分区：
文献类型：
--
作者：
Wei Wu;Shiyu Wang;Jianjun Wu;B. He;B. Zhu;L. Qin
内生细菌群落及其多样性与寄主的生长发育密切相关。本文研究了玉前、文县、磐安和平江4个产地苍术茎、叶、根和根茎中可培养的内生细菌及其与植物活性物质的潜在相关性。共分离鉴定出内生细菌118种，隶属于3门5纲11目26科48属。其中芽孢杆菌属为优势属。在AM中，组织类型和位置影响内生细菌群落。大约29.7%和28.8%的内生细菌分别表现出组织特异性和地理特异性。高效液相色谱分析表明，文县根茎中倍半萜（苍术内酯I、苍术内酯Ⅱ和苍术隆）含量高于平江、玉潜和磐安根茎。采用多元线性回归筛选与AM生物活性成分相关的菌株。微菌相对频率与AM中苍术内酯I和苍术内酯含量呈正相关，与苍术内酯Ⅱ含量呈负相关。该研究也为今后内生细菌作为植物次生代谢物替代来源的研究提供了理论框架。
The endophytic bacterial community and their diversity are closely related to the host's growth and development. This paper explores the culturable endophytic bacteria in the stems, leaves, roots and rhizomes of Atractylodes macrocephala (AM) of four localities (Yuqian, Wenxian, Pan'an and Pingjiang) and the potential correlation between the bacteria and plant bioactive compounds. A total of 118 endophytic bacteria belonging to 3 phyla, 5 classes, 11 orders, 26 families and 48 genera were isolated and identified from the four AM tissues. Among them, Bacillus was the dominant genus. In AM, the tissue type and locality influenced the endophytic bacterial community. Approximately 29.7 and 28.8% of the endophytic bacteria exhibited tissue specificity and geographic specificity, respectively. Furthermore, high-performance liquid chromatography revealed that the sesquiterpenoid (atractylenolide I, atractylenolide Ⅱ and atractylon) content was more in the rhizomes of Wenxian than in those of Pingjiang, Yuqian and Pan'an. The multiple linear regression was used to screen the bacterial strains related to the bioactive compounds of AM. The relative frequency of Microbacterium positively correlated with atractylenolide I and atractylon content in AM but negatively correlated with atractylenolide Ⅱ content. The study also provides a theoretical framework for future research on endophytic bacteria as alternative sources of secondary plant metabolites.