Evolution of two-component quorum sensing systems.
Evolution of two-component quorum sensing systems.
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DOI：
10.1099/acmi.0.000303
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发表时间：
2022
影响因子：
--
通讯作者：
Koumandou VL
中科院分区：
文献类型：
--
作者：
Giannakara M;Koumandou VL
群体感应（QS）是一种细胞与细胞之间的通讯系统，通过检测称为自诱导物的小分子，使细菌能够根据其种群密度协调其基因表达。通过这种方式，细菌可以集体行动，启动生物发光，毒性和生物膜形成等过程。自身诱导物由受体检测，其中一些是双组分信号转导系统（TCS）的一部分，其包括（通常膜结合的）传感器组氨酸激酶（HK）和同源反应调节剂（RR）。不同的细菌类群使用不同的QS系统，它们的相对进化关系尚未得到广泛的研究。为了解决这个问题，我们使用了基因和基因组（KEGG）数据库的京都百科全书，以确定所有的QS HK和RR的一部分，TCS和检查他们的保护跨微生物类群。我们使用简单模块化结构研究工具（SMART）和KEGG数据库比较了不同家族的受体和反应调节剂中高度保守结构域的组合，我们还对每个家族以及所有家族进行了系统发育分析。不同QS系统在不同类群中的分布表明HK-RR配对的灵活性，并强调了进一步研究最丰富系统的必要性。对于QS受体和反应调节剂，我们的研究结果表明某些家族之间存在密切的进化关系，突出了一个共同的进化历史，可以为未来的应用提供信息，例如设计致病性QS系统的新型抑制剂。
Quorum sensing (QS) is a cell-to-cell communication system that enables bacteria to coordinate their gene expression depending on their population density, via the detection of small molecules called autoinducers. In this way bacteria can act collectively to initiate processes like bioluminescence, virulence and biofilm formation. Autoinducers are detected by receptors, some of which are part of two-component signal transduction systems (TCS), which comprise of a (usually membrane-bound) sensor histidine kinase (HK) and a cognate response regulator (RR). Different QS systems are used by different bacterial taxa, and their relative evolutionary relationships have not been extensively studied. To address this, we used the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to identify all the QS HKs and RRs that are part of TCSs and examined their conservation across microbial taxa. We compared the combinations of the highly conserved domains in the different families of receptors and response regulators using the Simple Modular Architecture Research Tool (SMART) and KEGG databases, and we also carried out phylogenetic analyses for each family, and all families together. The distribution of the different QS systems across taxa, indicates flexibility in HK–RR pairing and highlights the need for further study of the most abundant systems. For both the QS receptors and the response regulators, our results indicate close evolutionary relationships between certain families, highlighting a common evolutionary history which can inform future applications, such as the design of novel inhibitors for pathogenic QS systems.