Gene Expression is Highly Regulated in Early Developing Fruit of Apple
Gene Expression is Highly Regulated in Early Developing Fruit of Apple
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DOI：
10.1007/s11105-011-0300-y
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发表时间：
2011-12-01
影响因子：
2.1
通讯作者：
Korban, Schuyler S.
中科院分区：
文献类型：
--
作者：
Soria-Guerra, Ruth Elena;Rosales-Mendoza, Sergio;Korban, Schuyler S.
从34个来自不同发育阶段、不同基因类型、不同生物和非生物胁迫下的营养组织和生殖组织的cdna文库中获得了近40,000个序列以及阳性和阴性对照，建立了苹果寡核苷酸微阵列。利用该基因芯片研究了“金冠”、“嘎拉”和“富士”三个苹果品种果实发育早期的基因表达谱。确定了一组3348个基因，这些基因在三种不同的基因类型中表现出显著的差异表达谱。这组基因主要包括编码新陈代谢相关酶的基因和与细胞周期有关的基因。差异表达基因分为17个功能类别。实时定量逆转录聚合酶链式反应(qRT-PCR)证实了微阵列分析中检测到的大多数基因的差异表达，特别是那些涉及细胞分裂、细胞扩张和细胞放大的基因。在所研究的这些基因中，EF-1α和PrP在不同的苹果基因型中表现出差异表达，并在果实发育早期表现出调控作用。此外，共鉴定了12个苹果品种在果实发育早期的差异表达基因。这些基因包括编码氨基转移酶家族蛋白、DNAJ热休克、组蛋白、Rubisco激活酶和含有四肽(TPR)重复序列的蛋白。这一全基因组分析表明，参与苹果早期果实发育的基因在不同类型的苹果中受到高度调控。
An oligonucleotide-based microarray for apple was developed consisting of similar to 40,000 sequences, along with positive and negative controls, obtained from 34 cDNA libraries constructed from both vegetative and reproductive tissues at different stages of development, varying genotypes, and under different biotic and abiotic stresses. This apple microarray was used to investigate global gene expression profiles in early developing fruit of three apple genotypes, including "Golden Delicious", "Gala", and "Fuji". A set of 3,348 genes, exhibiting significant differential expression profiles among the three different genotypes, was identified. This set primarily included genes encoding enzymes involved in metabolism and genes related to cell cycle. Differentially expressed genes were grouped into 17 functional categories. Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) confirmed differential expression for most genes detected in the microarray analysis, particularly those involved in cell division, cell expansion, and cell enlargement. Among those genes investigated, EF-1 alpha and PRP exhibited differential expression in different apple genotypes and demonstrated a regulatory role during early fruit development. Moreover, a total of 12 differentially expressed genes were identified during early fruit development in three genotypes of apple. These included genes encoding for aminotransferase family protein, DnaJ heat shock, histone, rubisco activase, and tetratricopeptide (TPR) repeat containing protein. This genome-wide analysis suggested that genes engaged in early fruit development among different genotypes of apple are highly regulated.