Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high-throughput sequencing
Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high-throughput sequencing
复制标题
高通量测序测定黄血蜱不同发育阶段的微生物群落特征
DOI：
10.1002/jobm.201600557
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发表时间：
2017-04-01
影响因子：
3.1
通讯作者：
Cheng, Tianyin
中科院分区：
文献类型：
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作者：
Duan, Deyong;Cheng, Tianyin
本研究分析了黄血蜱（haemaphyysalis flava， H. flava）在卵、幼虫、若虫和成虫四个发育阶段的体内微生物群落结构和多样性差异，以确定在黄血蜱不同发育阶段哪些细菌属可以通过经卵巢传播繁殖，哪些细菌属可以通过经卵巢传播传播。利用Illumina MiSeq测序平台对4个发育阶段样品黄螺旋体16S核糖体DNA (16S rDNA) V3区进行配对端测序。黄粉蜱在4个发育阶段的工作分类单位（otu）为：卵89个，幼虫111个，若虫104个，雌成蜱106个。其中64个otu在黄芽孢杆菌的4个发育阶段具有高度相似性。在各发育阶段丰度最高的细菌属有立克次体、柯谢氏菌、假单胞菌、埃利希菌、埃希氏菌、不动杆菌、柠檬酸杆菌和铜杆菌。蜱螨若虫科希氏体丰度最高，雌蜱立克次体丰度最高。除卵外，所有发育阶段均检出葡萄球菌和沃尔巴克氏体。
In this study, we analyzed the in vivo microbial community structure and diversity differences of Haemaphysalis flava (H. flava) in four developmental stages (egg, larva, nymph, and adult) to determine which bacterial genera could be propagated through transovarial transmission and transmitted by transstadial transmission during different developmental stages of H. flava. Paired-end sequencing of the V3 region of the 16S ribosomal DNA (16S rDNA) of H. flava in four developmental stage samples was conducted using the Illumina MiSeq sequencing platform. The following operational taxonomic units (OTUs) of H. flava were obtained during the four developmental stages: 89 of egg, 111 of larva, 104 of nymph, and 106 of female adult tick. Sixty-four of these OTUs had high similarity in the four developmental stages of H. flava. Eight bacterial genera had the highest abundances in all developmental stages, namely, Rickettsia, Coxiella, Pseudomonas, Ehrlichia, Escherichia, Acinetobacter, Citrobacter, and Cupriavidus. The nymph had the highest abundance of Coxiella, and the female adult tick had the highest abundance of Rickettsia. Staphylococcus and Wolbachia were detected in all developmental stages except the egg.