Induction of IL-1 during hemodialysis: transmembrane passage of intact endotoxins (LPS).
Induction of IL-1 during hemodialysis: transmembrane passage of intact endotoxins (LPS).
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血液透析过程中 IL-1 的诱导：完整内毒素 (LPS) 的跨膜通道。
DOI：
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发表时间：
1990
影响因子：
19.6
通讯作者：
N. Haeffner‐cavaillon
中科院分区：
文献类型：
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作者：
Maryline Laude;Martine Caroff;Laurent Simard;Christian Pusineri;Michel D. Kazatchkine;N. Haeffner‐cavaillon
慢性血液透析患者体内循环单核细胞被触发产生白细胞介素-1 （IL-1）。在首次使用纤维素膜透析的患者中，IL-1的透析诱导与补体激活有关。IL-1生成的慢性刺激发生在高透性膜透析患者中，其机制尚未确定。本研究表明，在模拟体内血液透析的体外条件下，完整的细菌脂多糖（LPS）分子可以穿过cuprofan、AN69和聚砜膜。实验使用了从脑膜炎奈瑟菌和从睾酮假单胞菌中纯化的LPS，这是一种从临床使用的透析液中生长出来的细菌菌株。将LPS纯化至均匀并进行放射性标记。在透析的前五分钟内观察到3h标记LPS的跨膜传代。透析1小时后，在透析液室中回收了0.1%至1%的3h标记LPS。大量的脂多糖，占原来透析液中含量的40 - 70%，被吸收到高透性膜上。低剂量的脂多糖被吸收到库泊芬膜上。脂多糖吸收量随透析液中脂多糖浓度的降低而降低。从血室中回收的LPS显示出与用于污染透析液的LPS相同的分子量。生物化学检测到的LPS跨膜通过与鲎试剂（LAL）检测到的物质无关。然而，在高透性膜透析时，在血室中检测到il -1诱导活性，正如先前使用cuprofan膜的其他人所发现的那样。（摘要删节250字）
Circulating monocytes of patients undergoing chronic hemodialysis are triggered to produce interleukin-1 (IL-1) in vivo. Intradialytic induction of IL-1 is associated with complement activation in patients dialyzed with first-use cellulose membranes. Chronic stimulation of IL-1 production occurs because of an yet unidentified mechanism in patients dialyzed with high permeability membranes. The present study demonstrates that intact bacterial lipopolysaccharide (LPS) molecules may cross cuprophan, AN69 and polysulfone membranes under in vitro conditions simulating in vivo hemodialysis. The experiments used purified LPS from Neisseria meningitidis and LPS from Pseudomonas testosteroni, a bacterial strain grown out from a clinically used dialysate. LPS were purified to homogeneity and radiolabeled. Transmembrane passage of 3H-labeled LPS was observed within the first five minutes of dialysis. A total of 0.1 to 1% of 3H-labeled LPS were recovered in the dialysate compartment after one hour of dialysis. High amounts of LPS, representing 40 to 70% of the amount originally present in the dialysate, were absorbed onto high permeability membranes. Low amounts of LPS were absorbed onto cuprophan membranes. The amount of LPS absorbed decreased with the concentration of LPS in the dialysate. LPS recovered from the blood compartment exhibited the same molecular weight as that used to contaminate the dialysate. Biochemically detectable transmembrane passage of LPS was not associated with that of material detectable using the limulus amebocyte lysate (LAL) assay. An IL-1-inducing activity was, however, detected in the blood compartment upon dialysis with high permeability membranes, as previously found by others with cuprophan membranes.(ABSTRACT TRUNCATED AT 250 WORDS)
Interleukin-1 及其与血液透析患者的相关性。
DOI：
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发表时间：
1988
期刊：
Kidney international. Supplement
影响因子：
--
作者：
Dinarello,CA;Koch,KM;Shaldon,S
通讯作者：
Shaldon,S
C5a 诱导人白细胞介素 1. 与内毒素或干扰素-γ 具有协同作用。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Okusawa,S;Dinarello,CA;Yancey,KB;Endres,S;Lawley,TJ;Frank,MM;Burke,JF;Gelfand,JA
通讯作者：
Gelfand,JA