A GEFI collaborative exercise on DNA/RNA co-analysis and mRNA profiling interpretation
A GEFI collaborative exercise on DNA/RNA co-analysis and mRNA profiling interpretation
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DOI：
10.1016/j.fsigss.2017.09.018
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发表时间：
2017-12-01
期刊：
影响因子：
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通讯作者：
Robino, C.
中科院分区：
文献类型：
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作者：
Carnevali, E.;Lacerenza, D.;Robino, C.
涉及15个GEFI的DNA/RNA共分析和RNA细胞分型协作练习（ISFG的意大利工作组）实验室与荷兰法医研究所合作组织的。参与者收到：1）用于19重mRNA谱分析的PCR引物，以及多重中靶向的每种细胞类型的参考纯化PCR产物; 2）详细的DNA/RNA共提取、mRNA谱分析和结果解释的方案; 3）一组8种模拟法医染色剂（7种单一来源，1种是两种体液的混合物）。正如预期的那样，从皮肤污渍中提取的低模板DNA出现了随机效应，至于mRNA谱，正确识别血液、唾液、阴道粘膜、精液和皮肤体液的实验室百分比>= 60%。成功率
A collaborative exercise on DNA/RNA co-analysis and RNA cell typing involving 15 GEFI (Italian working group of ISFG) laboratories was organized in collaboration with the Netherlands Forensic Institute.Participants received: 1) PCR primers for a 19-plex mRNA profiling assay, with reference purified PCR products for each cell type targeted in the multiplex; 2) detailed protocols for DNA/RNA co-extraction, mRNA profiling, and interpretation of results; 3) a set of 8 mock forensic stains (7 single source, one a mixture of two body fluids).All but one laboratory generated correct DNA typing results. As expected, stochastic effects were seen for low template DNA extracted from a skin stain.As for mRNA profiling, the percentage of laboratories that correctly identified body fluids was >= 60% for blood, saliva, vaginal mucosa, semen, and skin. Success rates were