Adjuvant activity of the heat-labile enterotoxin from enterotoxigenic Escherichia coli for oral administration of inactivated influenza virus vaccine
Adjuvant activity of the heat-labile enterotoxin from enterotoxigenic Escherichia coli for oral administration of inactivated influenza virus vaccine
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DOI：
10.1093/infdis/175.2.352
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发表时间：
1997-02-01
影响因子：
6.4
通讯作者：
Galphin, JC
中科院分区：
文献类型：
--
作者：
Katz, JM;Lu, XH;Galphin, JC
该研究表明，口服含有产肠毒素大肠杆菌热不稳定肠毒素（LT）的灭活流感疫苗，在BALB/c小鼠中引发了体液和细胞介导的反应谱，这对流感病毒感染的保护和恢复至关重要。与单独口服流感疫苗相比，LT与口服流感疫苗联合使用可增加血清抗病毒IgG和粘膜IgA反应，血清血凝抑制和中和抗体也被LT增强。佐剂增强了小鼠下呼吸道和上呼吸道对甲型流感H3N2病毒感染的保护作用，使口服疫苗使用剂量更低。LT与口服灭活流感疫苗联合使用可诱导流感病毒特异性增殖性T细胞、白细胞介素-2的产生和主要组织相容性复合体i类限制性细胞毒性T细胞。
Alternative strategies for vaccination against influenza that elicit both systemic antibody and mucosal IgA responses are needed to improve the efficacy in protection against infection, This study demonstrated that oral delivery of inactivated influenza vaccine with the heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli elicited the spectrum of humoral and cell-mediated responses in BALB/c mice critical for the protection and recovery from influenza virus infection, Coadministration of LT with oral influenza vaccine increased antiviral serum IgG and mucosal IgA responses compared with administration of oral influenza vaccine alone, Serum hemagglutination-inhibition and neutralizing antibodies were also augmented by LT, The adjuvant potentiated protection from infection with influenza A H3N2 viruses in mouse lower and upper respiratory tracts, enabling the use of lower doses of oral vaccine, Coadministration of LT with oral inactivated influenza vaccine induced influenza virus-specific proliferative T cells, interleukin-2 production, and major histocompatibility complex class I-restricted cytotoxic T cells.