Construction of a Genetic Map and Development of DNA Markers Linked to the Sex-Determining Locus in the Patagonian Pejerrey (Odontesthes hatcheri)
Construction of a Genetic Map and Development of DNA Markers Linked to the Sex-Determining Locus in the Patagonian Pejerrey (Odontesthes hatcheri)
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DOI：
10.1007/s10126-009-9194-1
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发表时间：
2010-02
影响因子：
3
通讯作者：
E. Koshimizu;C. A. Strüssmann;N. Okamoto;H. Fukuda;T. Sakamoto
中科院分区：
文献类型：
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作者：
E. Koshimizu;C. A. Strüssmann;N. Okamoto;H. Fukuda;T. Sakamoto
鱼类的性别分化过程受到遗传和环境因素的调节。巴塔哥尼亚斑龟 (Odontesthes hateri) 的性别似乎受到强烈的基因型控制 (GSD)，因为性别比例在 17°C 至 23°C 之间保持平衡 (1:1)。然而，在 <15°C 时，性别比例变得偏雌性，而在 25°C 时，性别比例则偏向雄性，这表明该物种也具有一定程度的温度依赖性性别决定（TSD）。个体遗传性别的鉴定将有助于阐明该物种性别分化的分子基础。在这项研究中，我们使用扩增片段长度多态性 (AFLP) 分析来开发两性的遗传连锁图谱以及巴塔哥尼亚佩杰瑞犬的性别连锁 DNA 标记。通过 64 个引物组合对 23 个雄性和 23 个雌性后代进行 AFLP 分析，总共产生了 153 个条带。雄性和雌性的遗传连锁图分别由20个连锁群中的79个标记和15个连锁群中的48个标记组成。鉴定出一种与性别决定位点紧密连锁的 AFLP 标记：标记 ACG/CAA-217，被扩增至男性特异性 DNA 片段。该区域的序列分析揭示了男性和女性之间的单核苷酸多态性（SNP），并将其转化为 SNP 标记。该标记提供了遗传证实，证明巴塔哥尼亚佩杰瑞犬的性别是由遗传决定的，并将有助于分析该物种中 GSD 和 TSD 的分子基础。
The process of sex differentiation in fishes is regulated by genetic and environmental factors. The sex of Patagonian pejerrey (Odontesthes hatcheri) appears to be under strong genotypic control (GSD) because the sex ratios are balanced (1:1) between 17°C and 23°C. However, sex ratios become female-biased at <15°C and male-biased at 25°C, which shows that this species also possesses some degree of temperature-dependent sex determination (TSD). Identification of the genetic sex of an individual will help elucidate the molecular basis of sex differentiation in this species. In this study, we used amplified fragment length polymorphism (AFLP) analysis to develop a genetic linkage map for both sexes and a sex-linked DNA marker for Patagonian pejerrey. The AFLP analysis of 23 male and 23 female progeny via 64 primer combinations produced a total of 153 bands. The genetic linkage map consisted of 79 markers in 20 linkage groups and 48 markers in 15 linkage groups for males and females, respectively. One AFLP marker tightly linked to the sex-determining locus was identified: the marker, ACG/CAA-217, amplified to the male-specific DNA fragment. Sequence analysis of this region revealed a single nucleotide polymorphism (SNP) between males and females, which was converted into a SNP marker. This marker provides genetic confirmation that the sex of Patagonian pejerrey is determined genetically and would be useful for the analysis of the molecular basis of GSD and TSD in this species.