Mitochondrial DNA depletion causes morphological changes in the mitochondrial reticulum of cultured human cells
Mitochondrial DNA depletion causes morphological changes in the mitochondrial reticulum of cultured human cells
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DOI：
10.1016/s0014-5793(00)01527-1
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发表时间：
2000-05-26
期刊：
影响因子：
3.5
通讯作者：
Selker, JML
中科院分区：
文献类型：
--
作者：
Gilkerson, RW;Margineantu, DH;Selker, JML
线粒体DNA（mtDNA）的消耗导致呼吸活动和能量产生的缺陷。最近的研究表明，线粒体主要存在的网状网络，具有管状嵴，使用荧光显微镜和透射电子显微镜，我们已经研究了线粒体的形态和内部结构在野生型和线粒体DNA缺失的p0人成纤维细胞和143B骨肉瘤细胞系。线粒体DNA缺失导致线粒体连续体的妥协，并导致嵴膜的量减少，通常促使剩余的嵴在线粒体内部采用圆形外观。这些变化强调了线粒体结构和功能之间的紧密关系。(C)2000年欧洲生物化学学会联合会。
Depletion of mitochondrial DNA (mtDNA) causes defects in respiratory activity and energy production. Recent studies have shown mitochondria to exist primarily as reticular networks, having tubular cristae, Using fluorescence microscopy and transmission electron microscopy, we have examined mitochondrial morphology and interior structure in wildtype and mtDNA-depleted p0 human fibroblasts and 143B osteosarcoma cell lines. MtDNA depletion results in compromise of the mitochondrial continuum and causes a reduction in amount of cristal membranes, often prompting the remaining cristae to adopt a circular appearance in the mitochondrial interior. These changes emphasize the tight relationship between mitochondrial structure and function. (C) 2000 Federation of European Biochemical Societies.