Molecular epidemiology of norovirus associated with gastroenteritis and emergence of norovirus GII.4 variant 2012 in Japanese pediatric patients
Molecular epidemiology of norovirus associated with gastroenteritis and emergence of norovirus GII.4 variant 2012 in Japanese pediatric patients
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DOI：
10.1016/j.meegid.2014.01.030
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发表时间：
2014-04-01
影响因子：
3.2
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Thongprachum, Aksara;Chan-it, Wisoot;Ushijima, Hiroshi
2012年底，由诺如病毒变异株悉尼_2012引起的急性胃肠炎暴发，许多国家都有报告。在这项研究中，我们描述了与胃肠炎相关的日本儿科患者诺沃克病毒感染发生率的监测研究，并调查了新的变种悉尼_2012在日本人群中传播的抗原变化。对2009年至2013年在北海道、东京、静冈、京都、大阪和佐贺收集的2381例急性胃肠炎儿童的粪便标本进行了诺沃克病毒检测和进一步的分子分析。检出诺如病毒阳性标本的比例较高(39.3%)，并检出多种基因型别。诺如病毒GII-4占优势(71.4%)。Den_HAAG_2006b(43.2%)被检测为主要变异，并与新奥尔良_2009(17.8%)共同传播至2012年3月。随后，他们被悉尼2012年取代。悉尼_2012变种是2012-2013年诺沃克病毒感染的主要原因(85.7%)。虽然悉尼_2012变异体与新奥尔良_2009变异体有共同的祖先，但对P2亚区的分析表明，与其他变异体相比，在抗原位点的四个氨基酸变化上具有较高的多样性。新变异体的特定残基393的变化可能会影响HBGA的识别。对过去4年流行的诺如病毒的分析表明，诺如病毒GII-4的优势变异在每个流行季节都有变化。推测表位氨基酸的变化可能导致病毒逃避现有的群体免疫，并解释了新变异暴发的增加。(C)2014爱思唯尔B.V.保留所有权利。
In late 2012, an outbreak of acute gastroenteritis due to norovirus variant Sydney_2012 occurred and have been reported from many counties. In this study, we described surveillance study of the incidence of norovirus infections among Japanese pediatric patients in association with gastroenteritis and investigated the antigenic change of the new variant Sydney_2012 circulated in Japanese populations. A total of 2381 fecal specimens collected from children with acute gastroenteritis in Hokkaido, Tokyo, Shizuoka, Kyoto, Osaka, and Saga from 2009 to 2013 were examined for norovirus and further analyzed molecularly. A high proportion (39.3%) of norovirus positive samples and several genotypes were detected. Norovirus GII.4 dominated over other genotypes (71.4%). The Den_Haag_2006b (43.2%) was detected as the predominant variant and co-circulated with New_Orleans_2009 (17.8%) until March 2012. Subsequently, they were displaced by Sydney_2012. The Sydney_2012 variant has been responsible for the majority of norovirus infections in 2012-2013 (85.7%). Although Sydney_2012 variant has a common ancestor with New Orleans_2009 variant, analysis of P2 sub-domain showed a high level of diversity in comparison with other variants in four amino acid changes at the antigenic sites. The change in particular residue 393 of new variant may affect HBGA recognition. Analysis of noroviruses circulating in the past 4 years revealed a change of predominant variant of norovirus GII.4 in each epidemic season. The change of amino acid in putative epitopes may have led the virus escape from the existing herd immunity and explain the increase of new variant outbreaks. (C)2014 Elsevier B.V. All rights reserved.