PRODUCTION OF COLOMINIC ACID BY PASTEURELLA-HAEMOLYTICA SEROTYPE-A2 ORGANISMS
PRODUCTION OF COLOMINIC ACID BY PASTEURELLA-HAEMOLYTICA SEROTYPE-A2 ORGANISMS
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DOI：
10.1111/j.1574-6968.1987.tb02293.x
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发表时间：
1987-06-01
影响因子：
2.1
通讯作者：
LINDON, JC
中科院分区：
文献类型：
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作者：
ADLAM, C;KNIGHTS, JM;LINDON, JC
经化学分析和~(13)C核磁共振波谱分析，从溶血性巴氏杆菌A2菌株的培养上清液中提纯的包膜多糖由(2-→，8)-α连接的N-乙酰神经氨酸聚合物组成。这与脑膜炎奈瑟氏菌B群和大肠杆菌K1的荚膜多糖相同，被称为Colominic酸。从第二个菌株分离的聚合物被α-1，4-连接的葡聚糖污染。众所周知，这两种聚合物的免疫原性很差，这解释了其他人未能为这种重要的绵羊病原体生产有效的提取疫苗。
Using chemical analysis and13C-nuclear magnetic resonance (NMR) spectroscopy, capsular polysaccharide purified from culture supernatants of a strain ofPasteurella haemolyticaserotype A2 was shown to consist of a (2 → 8)-α-linked polymer ofN-acetylneuraminic acid. This is identical to the capsular polysaccharides ofNeisseria meningitidisgroup B andEscherichia coliK1, and is known as colominic acid. Polymer isolated from a second strain was contaminated with α-1,4-linked dextran. The known poor immunogenicity of these two polymers explains the failure by others to produce effective extract vaccines for this important ovine pathogen.