ISOLATION AND CHARACTERIZATION OF AN ESTABLISHED ENDOTHELIAL-CELL LINE FROM TRANSGENIC MOUSE HEMANGIOMAS
ISOLATION AND CHARACTERIZATION OF AN ESTABLISHED ENDOTHELIAL-CELL LINE FROM TRANSGENIC MOUSE HEMANGIOMAS
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DOI：
10.1016/0014-4827(91)90265-v
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发表时间：
1991-10-01
影响因子：
3.7
通讯作者：
BAUTCH, VL
中科院分区：
文献类型：
--
作者：
DUBOIS, NA;KOLPACK, LC;BAUTCH, VL
从转基因小鼠中多瘤早期区基因表达诱导的血管瘤中分离出鼠内皮细胞系。经过两次细胞分选使用乙酰化低密度脂蛋白与荧光标记（Dil-Ac-LDL），一个纯的人口内皮细胞已进行了超过60代的动物。细胞保留内皮细胞特性，例如融合时的特征性鹅卵石外观、接触抑制生长和Ac-LDL的主动摄取。表达分析表明，细胞表达多瘤转基因和血管性血友病因子，内皮细胞标志物。将培养的内皮细胞皮下注射到非转基因组织相容性小鼠或裸鼠中导致血管瘤形成，并通过细胞分选从这些继发性血管瘤中重新分离内皮细胞。该细胞系代表了来自转基因小鼠的鼠内皮细胞的可再生来源，其可以在体外和通过重新引入宿主中进行研究。
A murine endothelial cell line was isolated from hemangiomas induced by expression of the polyoma early region gene in transgenic mice. After two cell sortings using acetylated low-density lipoprotein with a fluorescent label (Dil-Ac-LDL), a pure population of endothelial cells has been carried for more than 60 passages from the animal. The cells retain endothelial cell properties such as a characteristic cobblestone appearance at confluency, contact-inhibited growth, and active uptake of Ac-LDL. Expression analysis shows that the cells express both the polyoma transgene and the von Willebrand factor, an endothelial cell marker. Subcutaneous injection of the cultured endothelial cells into nontransgenic histocompatible mice or nude mice led to hemangioma formation, and endothelial cells were reisolated by cell sorting from these secondary hemangiomas. This cell line represents a renewable source of murine endothelial cells derived from transgenic mice that can be studied bothin vitroand by reintroduction into a host.