The THERMOSENSITIVE MALE STERILE 1 Interacts with the BiPs via DnaJ Domain and Stimulates Their ATPase Enzyme Activities in Arabidopsis.
The THERMOSENSITIVE MALE STERILE 1 Interacts with the BiPs via DnaJ Domain and Stimulates Their ATPase Enzyme Activities in Arabidopsis.
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拟南芥中热敏雄性不育 1 通过 DnaJ 结构域与 BiP 相互作用并刺激其 ATP 酶活性
DOI：
10.1371/journal.pone.0132500
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen LQ
中科院分区：
文献类型：
--
作者：
Ma ZX;Leng YJ;Chen GX;Zhou PM;Ye D;Chen LQ
拟南芥TM 1编码一个与Hsp40蛋白AtERdj3A相同的热休克蛋白，在花粉管和其他植物组织的耐热性中发挥重要作用。尽管它对植物的生长和繁殖很重要，但人们对它在植物耐热性方面的机制知之甚少。本研究探讨了TM 1与植物体内Hsp70蛋白、结合免疫球蛋白(BIPS)的关系，以了解TM 1在植物耐热性中的分子机制。与这三个Bip基因类似，Tm1不仅受热休克诱导，还受二硫苏糖醇和L氮杂环丁酸的诱导，提示Tm1可能参与了未折叠蛋白的反应。萤火虫荧光素酶互补成像(LCI)、GST下拉和ATPase活性测定表明，TM1DNAJ结构域可与BiP1和BiP3相互作用，并能刺激其ATPase活性。此外，在bzip28 bzip60双突变体中，TM 1的表达水平降低。这些结果表明，TM 1可能位于bZIP28和bZIP60下游，参与植物的耐温性，可能通过与BIPS相互作用参与未折叠和错误折叠蛋白质的复性或降解。
The Arabidopsis TMS1 encodes a heat shock protein identical to the Hsp40 protein AtERdj3A and plays important roles in the thermotolerance of pollen tubes and other plant tissues. Despite its importance to plant growth and reproduction, little has been known about its mechanisms underlying thermotolerance of plants. In this study, the relationship between TMS1 and the Hsp70 proteins, Binding Immunoglobulin Proteins (BiPs) was explored to understand the molecular mechanisms of TMS1 in thermotolerance of plants. The expression of TMS1 was induced not only by heat shock, but also by dithiothreitol (DTT) and L-azetidine-2-carboxylic acid (AZC), similarly to the three BiP genes, indicating that TMS1 may be involved in unfolded protein response (UPR). The firefly luciferase complementary imaging (LCI), GST pull-down and ATPase enzyme activity assays demonstrated that the DnaJ domain of TMS1 could interact with BiP1 and BiP3, and could stimulate their ATPase enzyme activities. In addition, the expression level of TMS1 was reduced in the bzip28 bzip60 double mutant. These results suggest that TMS1 may function at the downstream of bZIP28 and bZIP60 and be involved in termotolerance of plants, possibly by participating in refolding or degradation of unfolded and misfolded proteins through interaction with the BiPs.