Effects of sodium fluoride on hyperactivation and Ca2+ signaling pathway in sperm from mice: an in vivo study
Effects of sodium fluoride on hyperactivation and Ca2+ signaling pathway in sperm from mice: an in vivo study
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氟化钠对小鼠精子过度激活和 Ca2 信号通路的影响：一项体内研究
DOI：
10.1007/s00204-009-0508-x
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发表时间：
2010-05-01
影响因子：
6.1
通讯作者：
Wang, Jundong
中科院分区：
文献类型：
--
作者：
Sun, Zilong;Niu, Ruiyan;Wang, Jundong
精子超活化是成功受精的关键;然而，氟（F）对精子超活化的影响仍处于起步阶段。本研究旨在探讨氟化钠（NaF）对小鼠精子超活化、Ca 2 +/CALM-CAMK 2信号转导及CatSper 1和CatSper 2 mRNA表达的影响。成年雄性昆明小鼠经饮水给予30、70和150 mg NaF/l（相当于每天2.84 +/-A0.29、6.28 +/-A0.61和14.18 +/-A1.00 mg F/kg体重），连续49天。结果表明，NaF对精子的超激活运动有剂量依赖性的抑制作用。与对照组相比，70和150 mg NaF/l处理的小鼠细胞内Ca 2+浓度和CAMK 2蛋白显著降低，而在所有处理组中均未确定对CALM的影响。此外，与对照组相比，在中剂量和高剂量NaF（70，150 mg/l）下也观察到精子CatSper 1 mRNA表达降低，而在NaF给药组中未检测到CatSper 2 mRNA表达的变化。30 mg/l NaF处理对上述指标影响较小，无统计学差异。这些发现表明，暴露于70和150 mg/l NaF 49天，可通过改变涉及小鼠精子CatSper 1的Ca 2+信号通路导致低超活化。
Sperm hyperactivation is crucial for a successful fertilization; however, the influence of fluoride (F) to hyperactivation is still in its infancy. The purpose of this study was to investigate the effect of sodium fluoride (NaF) on sperm hyperactivation, Ca2+/CALM-CAMK2 signaling, and CatSper1 and CatSper2 mRNA expression in mice sperm. Adult male Kunming mice were administrated with 30, 70, and 150 mg NaF/l (corresponding to 2.84 +/- A 0.29, 6.28 +/- A 0.61, and 14.18 +/- A 1.00 mg F/kg body weight per day) through drinking water for 49 days. The results showed that NaF reduced the sperm hyperactivated motility in a dose-dependent manner. Compared with the controls, intracellular Ca2+ concentration and CAMK2 protein were significantly decreased in mice treated with 70 and 150 mg NaF/l, while no effect on CALM was determined in all treatment groups. Furthermore, decreased sperm CatSper1 mRNA expression was also observed in response to middle and higher doses of NaF (70, 150 mg/l) with comparison to the control group, whereas no change in the mRNA expression of CatSper2 was detected in NaF administrated groups. Treatment with 30 mg NaF/l exhibited slight effects on the above indexes with no statistical difference. These findings indicated that exposure to 70 and 150 mg/l NaF for 49 days could result in low hyperactivation via alteration of Ca2+ signaling pathway involving CatSper1 in sperm from mice.