Role of bacteria in modifying virulence of Entamoeba histolytica. Studies of amebae from axenic cultures.
Role of bacteria in modifying virulence of Entamoeba histolytica. Studies of amebae from axenic cultures.
复制标题
细菌在改变溶组织内阿米巴毒力中的作用。
DOI：
10.4269/ajtmh.1970.19.755
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
R. Rosenbaum
中科院分区：
文献类型：
--
作者：
M. Wittner;R. Rosenbaum
摘要
株大肠研究了来自纯培养物的溶组织菌的毒力和致病性。将来自无菌培养物的阿米巴原虫接种到断奶仓鼠的肝脏中不会导致肝脓肿。当阿米巴原虫和细菌在接种到肝脏前间隔约6至12小时重新结合时，阿米巴原虫与各种细菌菌株的重新结合会导致肝脓肿。然而，在axenization的毒力损失，发现下降缓慢，在一段时间内从3至5个月。为了确定是否有一些因素精心制作的，或与，细菌，将导致增加阿米巴毒力，我们添加过滤细菌培养物，细菌杀死的热或X射线辐射，或地面或超声处理的细菌，以阿米巴从无菌培养，然后接种到仓鼠或豚鼠的肝脏。这些方法均不能造成肝脓肿。同样，在由半透膜分隔的共生室中，无菌阿米巴和细菌的生长也不会导致阿米巴毒力增强。这些实验表明，毒力增加仅与阿米巴原虫与活细菌接触有关。可能有一种因子从细菌转移到阿米巴上，从而改变了毒力。
Abstract
Strains of E. histolytica from axenic cultures were studied with regard to their virulence and pathogenicity. Inoculation of amebae from axenic cultures into the liver of weanling hamsters did not result in hepatic abscesses. Reassociation of amebae with various bacterial strains resulted in hepatic abscess regularly when amebae and bacteria were reassociated for an interval of about 6 to 12 hours before inoculation into the liver. Loss of virulence upon axenization, however, was found to decline slowly over a period of from 3 to 5 months. In order to determine if there was some factor elaborated by, or associated with, bacteria that would cause increased amebic virulence, we added filtered bacterial cultures, bacteria killed by heat or X-radiation, or ground or sonicated bacteria to amebae from axenic cultures, which were then inoculated into livers of hamsters or guinea pigs. Hepatic abscess could not be produced by these methods. Similarly, growth of axenic amebae and bacteria in parabiotic chambers separated by a semipermeable membrane did not result in enhanced amebic virulence. These experiments suggest that increased virulence is associated only with contact of amebae with live bacteria. There may be the transfer of a factor from the bacteria to the amebae that alters virulence.