Antibiofilm activity of shikonin against Listeria monocytogenes and inhibition of key virulence factors
Antibiofilm activity of shikonin against Listeria monocytogenes and inhibition of key virulence factors
复制标题
紫草素对单核细胞增生李斯特氏菌的抗菌膜活性及对关键毒力因子的抑制
DOI：
10.1016/j.foodcont.2020.107558
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发表时间：
2021-02-01
期刊：
影响因子：
6
通讯作者：
Shi, Chao
中科院分区：
文献类型：
--
作者：
Li, Jiahui;Li, Siqi;Shi, Chao
紫草素是一种存在于紫草根中的生物活性物质，具有抗菌活性。本研究旨在检测紫草素对单核细胞增多性李斯特菌（Listeria monocytogenes）的膜抑制作用，并探讨紫草素对关键性李斯特菌（L.与人类感染相关的单核细胞增多症毒力因子。目前的研究结果表明，紫草素对L.单核细胞增多症的范围为25至100 μ g/mL。结晶紫染色证实，紫草素的亚抑制浓度（SICs）显着降低生物膜生物量在短短24小时。降低生物膜粘附，改变生物膜形态，和破坏的生物膜结构与紫草素的SICs治疗后，也证实了光学显微镜和场发射扫描电子显微镜。此外，在SICs，紫草素有效地降低了L。单核细胞增多症粘附和侵袭HT-29细胞，并阻止了溶血素O的分泌。此外，紫草素还抑制了SICs中与生物膜相关的基因和关键毒力基因的转录。因此，本研究结果表明紫草素可作为一种替代抗菌剂，以打击L。单核细胞增生生物膜形成和感染。
Shikonin is a bioactive compound found in the roots of Lithospermum erythrorhizon and has demonstrated antibacterial activity. In the current study, we aimed to examine the antibiofilm activity of shikonin against Listeria monocytogenes, and to investigate its effects on key L. monocytogenes virulence factors associated with human infection. Present results showed that the minimum inhibitory concentration of shikonin against L. monocytogenes ranged from 25 to 100 mu g/mL. Crystal violet staining confirmed that sub-inhibitory concentrations (SICs) of shikonin significantly reduced biofilm biomass in as little as 24 h. Decreased biofilm adhesion, altered biofilm morphology, and disruption of biofilm architecture following treatment with SICs of shikonin were also confirmed by light microscopy and field-emission scanning electron microscopy. Further, at SICs, shikonin effectively reduced the ability of L. monocytogenes to adhere to and invade HT-29 cells and prevented the secretion of listeriolysin O. Moreover, the transcription of biofilm-associated genes and key virulence genes that contribute to infection was also suppressed by shikonin at SICs. Therefore, present results suggest that shikonin could be used as an alternative antibacterial agent to combat L. monocytogenes biofilm formation and infection.