Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F).
Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F).
复制标题
葡萄糖代谢异常伴随着葡萄糖无法刺激大鼠胰腺细胞系（RINm5F）释放胰岛素。
作者：
P. Halban;G. A. Praz;C. Wollheim
本文研究了大鼠胰岛和大鼠胰岛素分泌细胞系(RINm5F)的葡萄糖代谢和胰岛素释放。完整的胰岛显示了葡萄糖利用的两个组成部分，葡萄糖刺激胰岛素释放与高KM成分(反映葡糖激酶样活性)有关。葡萄糖未能刺激RINm5F细胞释放胰岛素，RINm5F细胞只显示出葡萄糖利用的单一低Km成分。在被破坏的RINm5F细胞中只发现了低KM(己糖激酶样)葡萄糖磷酸化活性。葡萄糖代谢的这些变化可能是导致葡萄糖无法刺激RINm5F细胞释放胰岛素的原因之一。
Glucose metabolism and insulin release were studied in isolated rat islets and in an insulin-producing rat cell-line (RINm5F). Intact islets displayed two components of glucose utilization, with glucose stimulation of insulin release being associated with the high-Km component (reflecting glucokinase-like activity). Glucose failed to stimulate insulin release from RINm5F cells, which only displayed a single low-Km component of glucose utilization. Only low-Km (hexokinase-like) glucose-phosphorylating activity was found for disrupted RINm5F cells. These changes in glucose metabolism may contribute towards the failure of glucose to stimulate insulin release from RINm5F cells.